Overview
- External ontology
- TOGO:M1652 — Green Sulfur Bacterium Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1652 Original source: NBRC - NBRC_M855 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=855
Composition
flowchart LR
CultureMech_008209["green_sulfur_bacterium_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008209 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008209 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008209 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008209 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008209 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_008209 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_KCl["KCl"]
CultureMech_008209 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaHCO3["NaHCO3"]
CultureMech_008209 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_Ammonium_acetate["Ammonium acetate"]
CultureMech_008209 --> ing8_Ammonium_acetate
CHEBI_62947["CHEBI:62947"]
ing8_Ammonium_acetate -.-> CHEBI_62947
ing9_Na2S2O3__if_needed_["Na2S2O3 (if needed)"]
CultureMech_008209 --> ing9_Na2S2O3__if_needed_
ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008209 --> ing10_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing10_Carbon_dioxide_gas -.-> CHEBI_16526
ing11_Nitrogen_gas["Nitrogen gas"]
CultureMech_008209 --> ing11_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing11_Nitrogen_gas -.-> CHEBI_17997
ing12_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008209 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_008209 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008209 --> ing14_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing14_MnCl2_4H2O -.-> CHEBI_86368
ing15_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008209 --> ing15_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing15_CoCl2_6H2O -.-> CHEBI_35696
ing16_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008209 --> ing16_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_6H2O -.-> CHEBI_34887
ing17_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008209 --> ing17_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing17_ZnSO4_7H2O -.-> CHEBI_32312
ing18_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008209 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_Fructose["Fructose"]
CultureMech_008209 --> ing19_Fructose
CHEBI_28645["CHEBI:28645"]
ing19_Fructose -.-> CHEBI_28645
ing20_Magnesium_acetate["Magnesium acetate"]
CultureMech_008209 --> ing20_Magnesium_acetate
CHEBI_62964["CHEBI:62964"]
ing20_Magnesium_acetate -.-> CHEBI_62964
ing21_N2["N2"]
CultureMech_008209 --> ing21_N2
ing21_N2 -.-> CHEBI_17997
ing22_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008209 --> ing22_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing22_Na2S_9H2O -.-> CHEBI_76209
ing23_H2SO4["H2SO4"]
CultureMech_008209 --> ing23_H2SO4
CHEBI_26836["CHEBI:26836"]
ing23_H2SO4 -.-> CHEBI_26836
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 4 identical duplicates: 1.0, 1.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.5 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.025 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Ammonium acetate
C2H3O2.H4N 77.083 g/mol
|
25.5 G_PER_L |
CHEBI:62947
ammonium acetate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component [Merged 2 duplicates: 0.5, 25.0] |
| 10 | Na2S2O3 (if needed) | 0.5 G_PER_L | Properties: Defined component, Inorganic compound, Simple component | |
| 11 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 12 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 13 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.2 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
Fructose
C6H12O6 180.156 g/mol
|
25 G_PER_L |
CHEBI:28645
beta-D-fructofuranose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 21 |
Magnesium acetate
2C2H3O2.Mg 142.393 g/mol
|
25 G_PER_L |
CHEBI:62964
magnesium acetate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 22 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 23 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
15 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
Solutions
Vitamin B12 solution (0.001%)
Dose: 2 G_PER_L
Role: Vitamin; Properties: Defined component, Organic compound, Solution
Ferric citrate solution (0.1%)*
Dose: 5 G_PER_L
Role: Mineral source; Properties: Organic compound, Defined component, Solution
Trace element solution SL-6**
Dose: 2 G_PER_L
See mediadive_25_Trace_element_solution_SL-6.yaml for composition
Acetate solution***
Dose: 10 G_PER_L
Na2S·9H2O (neutralized)****
Dose: 0.6 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.057647Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1652 |
| 2026-01-28T19:48:14.318686Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: H2SO4 |
| 2026-03-09T08:04:23.804003+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T03:08:52.105079Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:54.484415Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008209 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.081226+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Green Sulfur Bacterium Medium" to "green_sulfur_bacterium_medium" for consistent matching |
| 2026-05-16T09:03:35.984355+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:06.710898+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:47.582146+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:59.177684+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:09.494173+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |