sulfolobus_medium_b

CultureMech:008217 archaea COMPLEX LIQUID
Original name: Sulfolobus Medium (B)

Overview

External ontology
TOGO:M165 — Sulfolobus Medium (B)
Applications
  • Microbial cultivation
pH
3.5
Notes
TOGO M165 uses one liter of TOGO M156 Sulfolobus Medium and adjusts it to pH 3.5 with 10 N H2SO4.

Composition

flowchart LR
    CultureMech_008217["sulfolobus_medium_b"]
    ing0_H2SO4["H2SO4"]
    CultureMech_008217 --> ing0_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing0_H2SO4 -.-> CHEBI_26836

Ingredients (1)

# Ingredient Concentration Ontology Notes
1 H2SO4
H2O4S 98.079 g/mol
10 N VARIABLE CHEBI:26836
sulfuric acid
TOGO lists 10 N H2SO4 for final pH adjustment. Normality is retained verbatim because the schema has no normality unit.

Preparation

  1. Use one liter of Sulfolobus Medium from TOGO M156 and adjust to pH 3.5 with 10 N H2SO4.

Solutions

Sulfolobus medium (see Medium [M156])

Dose: 1 L

CultureMech:008118

TOGO M165 uses one liter of TOGO M156 Sulfolobus Medium as the base medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.545398Z togo-import Imported from TOGO Medium Source: JCM, ID: M165
2026-01-28T19:48:21.576319Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-09T08:04:36.086505+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:54.604372Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008217
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:13.206719+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sulfolobus Medium (B)" to "sulfolobus_medium_b" for consistent matching
2026-05-16T09:03:10.708402+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:33.814974+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-10T00:00:00-07:00 repair_archaea_ph_wrappers_score20.py RESOLVED_ARCHAEA_PH_WRAPPER_SCORE20 Adjusts TOGO M156 Sulfolobus Medium to pH 3.5 with 10 N H2SO4.