Overview
- External ontology
- TOGO:M1665 — 1/5 Marine Broth + 0.5% Sodium Pyruvate
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1665 Original source: NBRC - NBRC_M868 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=868
Composition
flowchart LR
CultureMech_008223["1_5_marine_broth_0_5_sodium_pyruvate"]
ing0_Distilled_water["Distilled water"]
CultureMech_008223 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008223 --> ing1_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing1_Sodium_pyruvate -.-> CHEBI_50144
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008223 --> ing2_Agar__if_needed_
ing3_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
CultureMech_008223 --> ing3_Bacto_Marine_Broth_2216__Difco_
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
200 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 3 | Agar (if needed) | 750 G_PER_L | Role: Solidifying component | |
| 4 | Bacto Marine Broth 2216 (Difco) | 7.5 G_PER_L | Properties: Undefined component, Complex component |
Solutions
Seawater*
Dose: 800 G_PER_L
See mediadive_1801_Seawater.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.092513Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1665 |
| 2026-01-28T19:48:28.222121Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:46.724803+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:54.635760Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008223 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.240859+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1/5 Marine Broth + 0.5% Sodium Pyruvate" to "1_5_marine_broth_0_5_sodium_pyruvate" for consistent matching |
| 2026-05-16T09:02:58.163676+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:15.631548+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |