Overview
- External ontology
- TOGO:M1667 — Medium for strain C/L2
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1667 Original source: NBRC - NBRC_M870 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=870
Composition
flowchart LR
CultureMech_008225["medium_for_strain_c_l2"]
ing0_Distilled_water["Distilled water"]
CultureMech_008225 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008225 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008225 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008225 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008225 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008225 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6_KCl["KCl"]
CultureMech_008225 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_Na2SO4["Na2SO4"]
CultureMech_008225 --> ing7_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing7_Na2SO4 -.-> CHEBI_32149
ing8_yeast_extract["yeast extract"]
CultureMech_008225 --> ing8_yeast_extract
ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008225 --> ing9_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing9_Carbon_dioxide_gas -.-> CHEBI_16526
ing10_N2["N2"]
CultureMech_008225 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_HCl__25__["HCl (25%)"]
CultureMech_008225 --> ing11_HCl__25__
ing12_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008225 --> ing12_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_2H2O -.-> CHEBI_75213
ing13_H3BO3["H3BO3"]
CultureMech_008225 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008225 --> ing14_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing14_FeSO4_7H2O -.-> CHEBI_75836
ing15_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008225 --> ing15_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing15_MnCl2_4H2O -.-> CHEBI_86368
ing16_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008225 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008225 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008225 --> ing18_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing18_ZnSO4_7H2O -.-> CHEBI_32312
ing19_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008225 --> ing19_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing19_CuCl2_2H2O -.-> CHEBI_86318
ing20_Na2SeO_5H2O["Na2SeO·5H2O"]
CultureMech_008225 --> ing20_Na2SeO_5H2O
CHEBI_48843["CHEBI:48843"]
ing20_Na2SeO_5H2O -.-> CHEBI_48843
ing21_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_008225 --> ing21_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing21_Na2WO4_2H2O -.-> CHEBI_63939
ing22_NaOH["NaOH"]
CultureMech_008225 --> ing22_NaOH
CHEBI_32145["CHEBI:32145"]
ing22_NaOH -.-> CHEBI_32145
ing23_4_Aminobenzoic_acid["4-Aminobenzoic acid"]
CultureMech_008225 --> ing23_4_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_4_Aminobenzoic_acid -.-> CHEBI_30753
ing24_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008225 --> ing24_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing24_Thiamine_HCl -.-> CHEBI_49105
ing25_Calcium_D____panthothenate["Calcium D(+)-panthothenate"]
CultureMech_008225 --> ing25_Calcium_D____panthothenate
ing26_Nicotinic_acid["Nicotinic acid"]
CultureMech_008225 --> ing26_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing26_Nicotinic_acid -.-> CHEBI_15940
ing27_Pyridoxine_dihydrochloride["Pyridoxine dihydrochloride"]
CultureMech_008225 --> ing27_Pyridoxine_dihydrochloride
CHEBI_189426["CHEBI:189426"]
ing27_Pyridoxine_dihydrochloride -.-> CHEBI_189426
ing28_D____Biotin["D(+)-Biotin"]
CultureMech_008225 --> ing28_D____Biotin
CHEBI_15956["CHEBI:15956"]
ing28_D____Biotin -.-> CHEBI_15956
ing29_Cyanocobalamin["Cyanocobalamin"]
CultureMech_008225 --> ing29_Cyanocobalamin
CHEBI_17439["CHEBI:17439"]
ing29_Cyanocobalamin -.-> CHEBI_17439
CultureMech_008225 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1290.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 7 duplicates: 1.0, 987.0, 1.0, 100.0, 100.0, 1.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | yeast extract | variable VARIABLE | Role: Nutrient source; Properties: Undefined component | |
| 10 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 12 | HCl (25%) | 12.5 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 13 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2100 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
Na2SeO·5H2O
2Na.O3Se 172.937 g/mol
|
6 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
8 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 24 |
4-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
4 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 25 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 26 | Calcium D(+)-panthothenate | 5 G_PER_L | Role: Vitamin; Properties: Organic compound, Defined component, Simple component | |
| 27 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
10 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 28 |
Pyridoxine dihydrochloride
C8H11NO3.2HCl 242.102 g/mol
|
15 G_PER_L |
CHEBI:189426
Pyridoxine dihydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 29 |
D(+)-Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
5 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
NaHCO3
CHO3.Na 84.006 g/mol
|
84 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
1.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Inorganic compound, Solution, Defined component |
Solutions
Trace element mixture*
Dose: 1 G_PER_L
Selenite-tungstate solution**
Dose: 1 G_PER_L
See mediadive_5543_Selenite-tungstate_solution.yaml for composition
Vitamin mixture***
Dose: 1 G_PER_L
See mediadive_5209_Vitamin_mixture.yaml for composition
Vitamin B12 solution****
Dose: 1 G_PER_L
See mediadive_6105_Vitamin_B12_solution.yaml for composition
Bicarbonate solution (1.0 M)*****
Dose: 30 G_PER_L
Sulfide solution******
Dose: 24 G_PER_L
sodium lactate solution
Dose: variable VARIABLE
Role: Carbon source; Properties: Defined component, Organic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.097879Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1667 |
| 2026-01-28T19:48:27.079616Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: sodium lactate solution; Added default concentration for ingredient: yeast extract; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:44.890867+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:09:15.213942Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:19:54.669220Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008225 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.274065+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for strain C/L2" to "medium_for_strain_c_l2" for consistent matching |
| 2026-05-16T09:04:20.266040+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.392843+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 25 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.412677+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:06.162722+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |