tryptic_soy_broth

Overview

External ontology

TOGO:M1668 — Tryptic Soy Broth

Applications

• Microbial cultivation

Notes

Source: https://togomedium.org/medium/M1668 Original source: NBRC - NBRC_M871 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=871 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Cross-Mech

MediaIngredientMech · CommunityMech · TaxonMech · TraitMech · HabitatMech · CMMMech

Composition

flowchart LR
    CultureMech_008226["tryptic_soy_broth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008226 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_008226 --> ing1_Pancreatic_digest_of_casein
    ing2_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_008226 --> ing2_Peptic_digest_of_soybean_meal
    ing3_Glucose["Glucose"]
    CultureMech_008226 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Sodium_chloride["Sodium chloride"]
    CultureMech_008226 --> ing4_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing4_Sodium_chloride -.-> CHEBI_26710
    ing5_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_008226 --> ing5_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing5_Dipotassium_phosphate -.-> CHEBI_131527
    ing6_Agar["Agar"]
    CultureMech_008226 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

Target organisms and growth evidence

These are curated observations and conditions for named organisms; they do not imply universal growth suitability.

• Preferred term

  Arthrobacter psychrolactophilus

  Term

  Id

  NCBITaxon:92442

  Label

  Arthrobacter psychrolactophilus

  Genome assembly id

  • GCA_003219795

  Strain

  B7; ATCC 700733; DSM 15612; JCM 12399; CIP 107481

  Growth metrics

  • Temperature celsius

    22.0

    Measurement conditions

    Source reports a 1.5-2.3 h doubling-time range in TSB without dextrose containing 0.5% or 1.0% soluble starch or maltose.

    Evidence

    • Reference

      PMID:15931519

      Supports

      SUPPORT

      Snippet

      grew with a doubling time of 1.5-2.3 h (22 degrees C)

      Explanation

      The PubMed-indexed abstract explicitly reports growth and a doubling-time range for Arthrobacter psychrolactophilus ATCC 700733 in the TSBWD starch/maltose variant.

    Is max attainment

    False

  Evidence

  • Reference

    PMID:15931519

    Supports

    SUPPORT

    Explanation

    The study names Arthrobacter psychrolactophilus ATCC 700733 and reports positive growth in the TSBWD starch/maltose formulation.

  • Reference

    BacDive:7598

    Supports

    SUPPORT

    Explanation

    BacDive identifies B7 as the type strain corresponding to ATCC 700733, DSM 15612, JCM 12399, and CIP 107481, with NCBI taxon 92442 and genome assembly GCA_003219795.

Recipe variants

• Name

  tsb_without_dextrose_plus_starch_or_maltose

  Description

  Tryptic Soy Broth without dextrose, supplemented with soluble starch or maltose as the fermentable substrate.

  Modifications

  • Omit dextrose/glucose from the parent Tryptic Soy Broth formulation.
  • Add 0.5% or 1.0% (w/v) soluble starch or maltose.

  Purpose

  Growth and extracellular amylase production by Arthrobacter psychrolactophilus ATCC 700733 at 22 degrees C.

  Evidence

  • Reference

    PMID:15931519

    Supports

    SUPPORT

    Snippet

    tryptic soy broth without dextrose (TSBWD) containing 0.5% or 1.0%

    Explanation

    Smith and Zahnley report the study-specific TSBWD variant and the starch/maltose supplement levels used for Arthrobacter psychrolactophilus ATCC 700733 growth.

Provenance