Overview
- External ontology
- TOGO:M1679 — LB Broth + 0.05%(w/v) Na2CO3
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1679 Original source: NBRC - NBRC_M884 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=884 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_008237["lb_broth_0_05_w_v_na2co3"]
ing0_Distilled_water["Distilled water"]
CultureMech_008237 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Agar__if_needed_["Agar (if needed)"]
CultureMech_008237 --> ing1_Agar__if_needed_
ing2_Tryptone["Tryptone"]
CultureMech_008237 --> ing2_Tryptone
ing3_Yeast_extract["Yeast extract"]
CultureMech_008237 --> ing3_Yeast_extract
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_008237 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 3 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 4 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Solutions
Na2CO3*
Dose: 0.5 G_PER_L
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.127360Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1679 |
| 2026-01-28T19:48:27.659232Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:45.762508+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:54.791001Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008237 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.406056+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LB Broth + 0.05%(w/v) Na2CO3" to "lb_broth_0_05_w_v_na2co3" for consistent matching |
| 2026-05-16T09:03:38.814197+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:10.846226+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |