Overview
- External ontology
- TOGO:M1680 — Nutrient Broth + 0.5%(w/v) Na2CO3
- Applications
-
- Microbial cultivation
- pH
- 10.5
- Notes
- TOGO M1680 imports NBRC Medium 885: 8 g Bacto Nutrient Broth from Difco, 5 g Na2CO3, 15 g agar if needed, and 1 L distilled water; the final pH is 10.5.
Composition
flowchart LR
CultureMech_008239["nutrient_broth_0_5_w_v_na2co3"]
ing0_Bacto_Nutrient_Broth__Difco_["Bacto Nutrient Broth (Difco)"]
CultureMech_008239 --> ing0_Bacto_Nutrient_Broth__Difco_
ing1_Na2CO3["Na2CO3"]
CultureMech_008239 --> ing1_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing1_Na2CO3 -.-> CHEBI_29377
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008239 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
ing3_Distilled_water["Distilled water"]
CultureMech_008239 --> ing3_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing3_Distilled_water -.-> CHEBI_15377
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto Nutrient Broth (Difco) | 8.0 G_PER_L | TOGO M1680 and NBRC Medium 885 list 8.0 g/L Bacto Nutrient Broth from Difco; the commercial product is retained without an ontology grounding. | |
| 2 |
Na2CO3
CO3.2Na 105.988 g/mol
|
5.0 G_PER_L |
CHEBI:29377
sodium carbonate
|
TOGO M1680 and NBRC Medium 885 list 5.0 g/L Na2CO3 for a 0.5% w/v alkaline supplement. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M1680 and NBRC Medium 885 list 15.0 g/L Agar if needed for solid medium. |
| 4 |
Distilled water
H2O 18.015 g/mol
|
1000.0 ML_PER_L |
CHEBI:15377
water
|
TOGO M1680 and NBRC Medium 885 list 1 L Distilled water. |
Preparation
- Suspend Bacto Nutrient Broth (Difco) and optional agar in distilled water.
- Autoclave the nutrient-broth agar base.
- Sterilize the Na2CO3 stock separately.
- Aseptically add the sterile Na2CO3 stock to the sterile base.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.128226Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1680 |
| 2026-01-28T19:48:24.755398Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.959269+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:54.798989Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008239 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:17.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.414564+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nutrient Broth + 0.5%(w/v) Na2CO3" to "nutrient_broth_0_5_w_v_na2co3" for consistent matching |
| 2026-05-16T09:04:27.921393+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.161474+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_nutrient_broths_score15.py | RESOLVED_TOGO_M1680_NBRC_885 | TOGO M1680 imports NBRC Medium 885: 8 g Bacto Nutrient Broth from Difco, 5 g Na2CO3, 15 g agar if needed, and 1 L distilled water; the final pH is 10.5. |