1_5_nutrient_agar

CultureMech:008251 bacterial COMPLEX SOLID_AGAR
Original name: 1/5 Nutrient agar

Overview

External ontology
TOGO:M1691 — 1/5 Nutrient agar
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1691 Original source: NBRC - NBRC_M898 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=898 Commercial Product: Nutrient Agar Supplier: Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Catalog: TM Media, BD 213000 Source: https://microbenotes.com/nutrient-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_008251["1_5_nutrient_agar"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008251 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Agar["Agar"]
    CultureMech_008251 --> ing1_Agar
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar -.-> CHEBI_2509
    ing2_Peptone["Peptone"]
    CultureMech_008251 --> ing2_Peptone
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_008251 --> ing3_Yeast_extract
    ing4_Beef_extract["Beef extract"]
    CultureMech_008251 --> ing4_Beef_extract
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_008251 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Agar["Agar"]
    CultureMech_008251 --> ing6_Agar
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Agar 14 G_PER_L CHEBI:2509
agar
Role: Solidifying component
3 Peptone
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
5.0 G_PER_L MICRO:0000178 (source link unavailable)
peptone
Enzymatic digest of protein, provides nitrogen, amino acids, and peptides; Constituent of Nutrient Agar commercial product
4 Yeast extract
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
1.5 G_PER_L FOODON:03315426
yeast extract
Provides B-complex vitamins and additional nutrients; Constituent of Nutrient Agar commercial product
5 Beef extract
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
1.5 G_PER_L FOODON:03302088
beef extract
Aqueous extract of beef tissue, provides organic compounds and minerals; Constituent of Nutrient Agar commercial product
6 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
5.0 G_PER_L CHEBI:26710
sodium chloride
Maintains osmotic equilibrium; Constituent of Nutrient Agar commercial product
7 Agar
Multiple suppliers (TM Media, BD, Sigma-Aldrich, HiMedia) Nutrient Agar TM Media, BD 213000
15.0 G_PER_L CHEBI:2509
agar
Solidifying agent. Omit for Nutrient Broth (liquid variant); Constituent of Nutrient Agar commercial product

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.145909Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1691
2026-01-28T19:48:18.457781Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:54.883033Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008251
2026-03-17T06:30:04.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:13.504544+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 Nutrient agar" to "1_5_nutrient_agar" for consistent matching
2026-06-05T07:40:15.642331+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.