Overview
- External ontology
- TOGO:M1696 — Reinforced Clostridial medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1696 imports NBRC Medium 902 as Reinforced Clostridial medium. NBRC 902 lists 38 g CM0149 Broth from OXOID, 1 L distilled water, optional 15 g agar, and autoclaving at 121 C for 15 min. The source does not state pH or incubation temperature.
Composition
flowchart LR
CultureMech_008256["reinforced_clostridial_medium"]
ing0_CM0149_Broth__OXOID_["CM0149 Broth (OXOID)"]
CultureMech_008256 --> ing0_CM0149_Broth__OXOID_
ing1_Distilled_water["Distilled water"]
CultureMech_008256 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008256 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | CM0149 Broth (OXOID) | 38.0 G_PER_L | NBRC Medium 902 lists 38 g/L CM0149 Broth from OXOID; this dehydrated commercial mixture is retained as an opaque complex component. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
NBRC Medium 902 lists 1 L distilled water. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
TOGO M1696 / NBRC Medium 902 lists 15.0 g/L Agar (if needed). |
Preparation
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.158614Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1696 |
| 2026-01-28T19:48:27.650513Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:54.941500Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008256 |
| 2026-03-17T06:30:23.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.565310+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Reinforced Clostridial medium" to "reinforced_clostridial_medium" for consistent matching |
| 2026-06-05T07:40:34.013538+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m1696_score15.py | RESOLVED_TOGO_M1696_SCORE15 | TOGO M1696 imports NBRC Medium 902 as Reinforced Clostridial medium. NBRC 902 lists 38 g CM0149 Broth from OXOID, 1 L distilled water, optional 15 g agar, and autoclaving at 121 C for 15 min. The source does not state pH or incubation temperature. Corrected the imported water g/L artifact, added the NBRC autoclave step, and marked the NBRC formula as curated. |