acidianus_brierleyi_medium

CultureMech:008260 archaea COMPLEX LIQUID
Original name: Acidianus Brierleyi Medium

Overview

External ontology
TOGO:M169 — Acidianus Brierleyi Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M169 Original source: JCM - JCM_M176 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=176

Composition

flowchart LR
    CultureMech_008260["acidianus_brierleyi_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008260 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008260 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_KH2PO4["KH2PO4"]
    CultureMech_008260 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_008260 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008260 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_Sulfur__powder_["Sulfur (powder)"]
    CultureMech_008260 --> ing5_Sulfur__powder_
    CHEBI_33403["CHEBI:33403"]
    ing5_Sulfur__powder_ -.-> CHEBI_33403
    ing6_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_008260 --> ing6_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing6_Ca_NO3_2 -.-> CHEBI_64205
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_008260 --> ing7_Yeast_extract__BD_Difco_
    ing8_H2SO4["H2SO4"]
    CultureMech_008260 --> ing8_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing8_H2SO4 -.-> CHEBI_26836

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
6 Sulfur (powder) 10 G_PER_L CHEBI:33403
elemental sulfur
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
7 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.01 G_PER_L CHEBI:64205
calcium nitrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 Yeast extract (BD-Difco) 0.2 G_PER_L Properties: Undefined component
9 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-06-14T05:58:58.561437+00:00 idnf-grounding-fix-v1.0 Fixed ID_NOT_FOUND term groundings Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable).
2026-01-27T06:35:54.547974Z togo-import Imported from TOGO Medium Source: JCM, ID: M169
2026-01-28T19:48:27.498398Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-13T06:19:54.991864Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008260
2026-03-17T06:30:23.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:13.617247+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Acidianus Brierleyi Medium" to "acidianus_brierleyi_medium" for consistent matching
2026-05-16T09:03:10.912386+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:34.019303+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:30.851398+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:24.226481+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.085157+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.