marine_broth_with_0_5_methanol

CultureMech:008300 bacterial COMPLEX SOLID_AGAR
Original name: Marine Broth with 0.5% Methanol

Overview

External ontology
TOGO:M1738 — Marine Broth with 0.5% Methanol
Applications
  • Microbial cultivation
Notes
NBRC Medium 947 Marine Broth with 0.5% Methanol lists 37.4 g Bacto Marine Broth 2216 (Difco), 5 ml methanol, 15 g optional agar, and 1 L distilled water with pH unadjusted. NBRC marks methanol for separate filter sterilization.

Composition

flowchart LR
    CultureMech_008300["marine_broth_with_0_5_methanol"]
    ing0_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
    CultureMech_008300 --> ing0_Bacto_Marine_Broth_2216__Difco_
    ing1_Methanol["Methanol"]
    CultureMech_008300 --> ing1_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing1_Methanol -.-> CHEBI_17790
    ing2_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008300 --> ing2_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing2_Agar__if_needed_ -.-> CHEBI_2509
    ing3_Distilled_water["Distilled water"]
    CultureMech_008300 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377

Ingredients (4)

# Ingredient Concentration Ontology Notes
1 Bacto Marine Broth 2216 (Difco) 37.4 G_PER_L NBRC Medium 947 lists 37.4 g/L Bacto Marine Broth 2216 from Difco without disclosing the product composition.
2 Methanol
CH4O 32.042 g/mol
5 ML_PER_L CHEBI:17790
methanol
 NBRC Medium 947 lists 5 ml/L methanol and marks it for separate filter sterilization.
3 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 NBRC Medium 947 lists 15 g/L agar as an optional solidifying agent.
4 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 NBRC Medium 947 lists 1 L distilled water.

Preparation

  1. Mix 37.4 g Bacto Marine Broth 2216 (Difco) in 1 L distilled water with 15 g agar if a solid medium is needed.
  2. Sterilize methanol separately by filtration.
  3. Add 5 ml filter-sterilized methanol per liter of medium.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.255884Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1738
2026-01-28T19:48:20.710632Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:34.343403+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:55.500244Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008300
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:09.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.048944+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Marine Broth with 0.5% Methanol" to "marine_broth_with_0_5_methanol" for consistent matching
2026-05-16T09:03:40.232255+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:12.874339+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1738_marine_methanol_score15.py RESOLVED_TOGO_M1738_MARINE_METHANOL_SCORE15 NBRC Medium 947 Marine Broth with 0.5% Methanol lists 37.4 g Bacto Marine Broth 2216 (Difco), 5 ml methanol, 15 g optional agar, and 1 L distilled water with pH unadjusted. NBRC marks methanol for separate filter sterilization. Corrected distilled water from 1 g/L to 1 L, converted methanol from an empty migrated solution to a 5 ml/L ingredient, grounded methanol, agar, and water, and restored NBRC's pH and filter-sterilization notes.