horikoshi_i_medium

CultureMech:008313 bacterial COMPLEX SOLID_AGAR
Original name: Horikoshi-I Medium

Overview

External ontology
TOGO:M174 — Horikoshi-I Medium
Applications
  • Microbial cultivation
pH
10.0
Notes
Source: TOGO Medium M174 | Link: https://togomedium.org/medium/M174 TOGO Medium M174 mirrors JCM Medium 181 Horikoshi-I medium: a 900 mL agar base with glucose, Polypepton, yeast extract, dipotassium hydrogen phosphate, magnesium sulfate heptahydrate, and agar plus 100 mL sterile 10% Na2CO3 solution after autoclaving, for a final pH of 10.0.

Composition

flowchart LR
    CultureMech_008313["horikoshi_i_medium"]
    ing0_Glucose["Glucose"]
    CultureMech_008313 --> ing0_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing0_Glucose -.-> CHEBI_17234
    ing1_Polypepton__Nihon_Pharm__Co__["Polypepton (Nihon Pharm. Co.)"]
    CultureMech_008313 --> ing1_Polypepton__Nihon_Pharm__Co__
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008313 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008313 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_008313 --> ing4_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing5_Agar["Agar"]
    CultureMech_008313 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_Distilled_water["Distilled water"]
    CultureMech_008313 --> ing6_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing6_Distilled_water -.-> CHEBI_15377

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
 JCM Medium 181 lists this component for Horikoshi-I medium.
2 Polypepton (Nihon Pharm. Co.) 5 G_PER_L JCM Medium 181 lists this component for Horikoshi-I medium.
3 Yeast extract 5 G_PER_L FOODON:03315426
yeast extract
 JCM Medium 181 lists this component for Horikoshi-I medium.
4 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 JCM Medium 181 lists this component for Horikoshi-I medium.
5 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 JCM Medium 181 lists this component for Horikoshi-I medium.
6 Agar 15 G_PER_L CHEBI:2509
agar
 JCM Medium 181 lists this component for Horikoshi-I medium.
7 Distilled water
H2O 18.015 g/mol
900 ML_PER_L CHEBI:15377
water
 TOGO Medium M174/JCM Medium 181 lists this distilled-water volume.

Preparation

  1. Prepare the Horikoshi-I agar base in 900 mL water.
  2. Autoclave at 121 C for 15 min.
  3. Aseptically add 100 mL of sterile 10% Na2CO3 solution.
  4. Check the final pH to be about 10.0.

Solutions

10% Na2CO3 solution

Dose: 100 ML_PER_L

Stock composition
IngredientConcentrationNotes
Na2CO3 10 PERCENT_W_V TOGO Medium M174/JCM Medium 181 uses this compound in a sterile 10% Na2CO3 solution.
TOGO Medium M174/JCM Medium 181 says to aseptically add 100 mL of sterile 10% Na2CO3 solution after autoclaving.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:54.555533Z togo-import Imported from TOGO Medium Source: JCM, ID: M174
2026-01-28T19:48:30.003482Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:49.865877+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:55.600490Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008313
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.139600+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Horikoshi-I Medium" to "horikoshi_i_medium" for consistent matching
2026-05-16T09:03:11.090609+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:34.200417+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:24.381292+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.144353+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py RESOLVED_JCM_SALINITY_SCORE20 Rebuilt the TOGO M174/JCM Medium 181 Horikoshi-I medium recipe.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M407_Horikoshi-I_Medium_With_2_NaCl.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M1092_Horikoshi-I_Medium_With_3.5_NaCl.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M404_Horikoshi-I_Medium_With_5_NaCl.yaml.
2026-09-08T00:00:00-07:00 repair_jcm_salinity_score20.py LINKED_JCM_SALINITY_SCORE20_CHILDREN Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M340_Horikoshi-1_Medium_With_10_NaCl.yaml.