Overview
- External ontology
- TOGO:M1759 — Pseudomonas saccharophila medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1759 Original source: NBRC - NBRC_M971 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=971
Composition
flowchart LR
CultureMech_008323["pseudomonas_saccharophila_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008323 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008323 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008323 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_NH4Cl["NH4Cl"]
CultureMech_008323 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_Na2HPO4["Na2HPO4"]
CultureMech_008323 --> ing4_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4 -.-> CHEBI_34683
ing5_Agar__if_needed_["Agar (if needed)"]
CultureMech_008323 --> ing5_Agar__if_needed_
ing6_CaCl2["CaCl2"]
CultureMech_008323 --> ing6_CaCl2
CHEBI_3312["CHEBI:3312"]
ing6_CaCl2 -.-> CHEBI_3312
ing7_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_008323 --> ing7_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing7_Ferric_ammonium_citrate -.-> CHEBI_31604
ing8_Sucrose["Sucrose"]
CultureMech_008323 --> ing8_Sucrose
CHEBI_17992["CHEBI:17992"]
ing8_Sucrose -.-> CHEBI_17992
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
982.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 3 duplicates: 980.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
4.8 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 7 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 | Ferric ammonium citrate | 5 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Organic compound |
| 9 |
Sucrose
C12H22O11 342.297 g/mol
|
100 G_PER_L |
CHEBI:17992
sucrose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
Solutions
Solution A*
Dose: 10 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B**
Dose: 10 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.294900Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1759 |
| 2026-01-28T19:48:35.732691Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:59.489553+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:31.234196Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:55.700552Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008323 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.241938+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pseudomonas saccharophila medium" to "pseudomonas_saccharophila_medium" for consistent matching |
| 2026-05-16T09:04:29.594493+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:42.747413+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:30.107489+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:10.480357+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |