Overview
- External ontology
- TOGO:M1761 — Medium for strain RASEN
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1761 Original source: NBRC - NBRC_M974 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=974
Composition
flowchart LR
CultureMech_008326["medium_for_strain_rasen"]
ing0_Distilled_water["Distilled water"]
CultureMech_008326 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008326 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008326 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008326 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008326 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_008326 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_008326 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008326 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008326 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_KCl["KCl"]
CultureMech_008326 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaHCO3["NaHCO3"]
CultureMech_008326 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008326 --> ing11_Bacto_Yeast_Extract__Difco_
ing12_Cysteine_HCl["Cysteine-HCl"]
CultureMech_008326 --> ing12_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing12_Cysteine_HCl -.-> CHEBI_91247
ing13_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008326 --> ing13_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing13_Carbon_dioxide_gas -.-> CHEBI_16526
ing14_Nitrogen_gas["Nitrogen gas"]
CultureMech_008326 --> ing14_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing14_Nitrogen_gas -.-> CHEBI_17997
ing15_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008326 --> ing15_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing15_Na2MoO4_2H2O -.-> CHEBI_75213
ing16_H3BO3["H3BO3"]
CultureMech_008326 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008326 --> ing17_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_4H2O -.-> CHEBI_86368
ing18_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008326 --> ing18_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing18_CoCl2_6H2O -.-> CHEBI_35696
ing19_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008326 --> ing19_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_6H2O -.-> CHEBI_34887
ing20_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008326 --> ing20_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_2H2O -.-> CHEBI_86318
ing21_ZnCl2["ZnCl2"]
CultureMech_008326 --> ing21_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing21_ZnCl2 -.-> CHEBI_49976
ing22_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008326 --> ing22_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing22_Nitrilotriacetic_acid -.-> CHEBI_44557
ing23_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_008326 --> ing23_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_2H2O -.-> CHEBI_63939
ing24_NaBr["NaBr"]
CultureMech_008326 --> ing24_NaBr
CHEBI_63004["CHEBI:63004"]
ing24_NaBr -.-> CHEBI_63004
ing25_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_008326 --> ing25_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing25_SrCl2_6H2O -.-> CHEBI_36385
ing26_KI["KI"]
CultureMech_008326 --> ing26_KI
CHEBI_8346["CHEBI:8346"]
ing26_KI -.-> CHEBI_8346
ing27_Na2SeO3["Na2SeO3"]
CultureMech_008326 --> ing27_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing27_Na2SeO3 -.-> CHEBI_48843
ing28_AlCl3["AlCl3"]
CultureMech_008326 --> ing28_AlCl3
CHEBI_30114["CHEBI:30114"]
ing28_AlCl3 -.-> CHEBI_30114
ing29_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_008326 --> ing29_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing29_FeCl2_4H2O -.-> CHEBI_86249
CultureMech_008326 --> more["...10 more"]
Ingredients (40)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 3 identical duplicates: 1.0, 1.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.06 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
70 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.535 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
90 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Bacto Yeast Extract (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 13 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.3 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Defined component, Simple component, Organic compound |
| 14 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 15 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 16 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
10 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.53 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.42 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
25 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
7 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
90 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
0.15 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 24 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.3 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NaBr
Br.Na 102.894 g/mol
|
10 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
10 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
KI
I.K 166.002 g/mol
|
10 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
0.2 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
AlCl3
AlCl3 133.341 g/mol
|
6 G_PER_L |
CHEBI:30114
aluminium trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.1 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 40 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace element solution*
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Vitamin solution**
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.298064Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1761 |
| 2026-01-28T19:48:31.861711Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:53.058961+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:24.491288Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:55.728979Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008326 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.271186+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for strain RASEN" to "medium_for_strain_rasen" for consistent matching |
| 2026-05-16T09:04:20.643301+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.771763+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.296940+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:06.311259+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:48.343187+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |