1_2_r2a_75_asw

CultureMech:008341 bacterial COMPLEX SOLID_AGAR
Original name: 1/2 R2A-75%ASW

Overview

External ontology
TOGO:M1775 — 1/2 R2A-75%ASW
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1775 Original source: NBRC - NBRC_M991 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=991

Composition

flowchart LR
    CultureMech_008341["1_2_r2a_75_asw"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008341 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Agar___if_needed_["Agar  (if needed)"]
    CultureMech_008341 --> ing1_Agar___if_needed_
    ing2_R2A_Broth__DAIGO_["R2A Broth (DAIGO)"]
    CultureMech_008341 --> ing2_R2A_Broth__DAIGO_
    ing3_NaCl["NaCl"]
    CultureMech_008341 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008341 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_H3BO3["H3BO3"]
    CultureMech_008341 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_KCl["KCl"]
    CultureMech_008341 --> ing6_KCl
    CHEBI_32588["CHEBI:32588"]
    ing6_KCl -.-> CHEBI_32588
    ing7_NaHCO3["NaHCO3"]
    CultureMech_008341 --> ing7_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing7_NaHCO3 -.-> CHEBI_32139
    ing8_Na2SO4["Na2SO4"]
    CultureMech_008341 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_NaF["NaF"]
    CultureMech_008341 --> ing9_NaF
    CHEBI_28741["CHEBI:28741"]
    ing9_NaF -.-> CHEBI_28741
    ing10_KBr["KBr"]
    CultureMech_008341 --> ing10_KBr
    CHEBI_32030["CHEBI:32030"]
    ing10_KBr -.-> CHEBI_32030
    ing11_SrCl2["SrCl2"]
    CultureMech_008341 --> ing11_SrCl2
    CHEBI_36383["CHEBI:36383"]
    ing11_SrCl2 -.-> CHEBI_36383
    ing12_MgCl2["MgCl2"]
    CultureMech_008341 --> ing12_MgCl2
    CHEBI_6636["CHEBI:6636"]
    ing12_MgCl2 -.-> CHEBI_6636

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
251.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 250.0, 1.0]
2 Agar (if needed) 15 G_PER_L Role: Solidifying component
3 R2A Broth (DAIGO) 1.6 G_PER_L Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
23.5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
1.1 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 H3BO3
H3BO3 61.833 g/mol
26 G_PER_L CHEBI:33118
boric acid
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 KCl
Cl.K 74.551 g/mol
0.66 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 NaHCO3
CHO3.Na 84.006 g/mol
0.19 G_PER_L CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
9 Na2SO4
2Na.O4S 142.043 g/mol
3.9 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
10 NaF
F.Na 41.988 g/mol
3 G_PER_L CHEBI:28741
sodium fluoride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 KBr
Br.K 119.002 g/mol
96 G_PER_L CHEBI:32030
potassium bromide
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
12 SrCl2
2Cl.Sr 158.526 g/mol
24 G_PER_L CHEBI:36383
strontium dichloride
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
13 MgCl2
2Cl.Mg 95.211 g/mol
4.9 G_PER_L CHEBI:6636
magnesium dichloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component

Solutions

Artificial sea water*

Dose: 750 G_PER_L

See mediadive_2170_Artificial_sea_water.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.332614Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1775
2026-01-28T19:48:34.141080Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:56.655875+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T03:09:28.233045Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:55.884244Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008341
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:37.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.428741+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/2 R2A-75%ASW" to "1_2_r2a_75_asw" for consistent matching
2026-05-16T09:02:58.074471+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:15.506377+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:42.490389+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.