Overview
- External ontology
- TOGO:M1776 — M1 Medium
- Applications
-
- Microbial cultivation
- Notes
- TOGO M1776 imports NBRC Medium 992 M1 Medium. NBRC 992 lists 10 g starch, 4 g yeast extract, 2 g peptone, 18 g agar if needed, and 1 L seawater, with seawater supplied as filtered aged seawater or artificial seawater such as Daigo's Artificial Seawater SP.
Composition
flowchart LR
CultureMech_008342["m1_medium"]
ing0_Starch["Starch"]
CultureMech_008342 --> ing0_Starch
CHEBI_28017["CHEBI:28017"]
ing0_Starch -.-> CHEBI_28017
ing1_Yeast_extract["Yeast extract"]
CultureMech_008342 --> ing1_Yeast_extract
ing2_Peptone["Peptone"]
CultureMech_008342 --> ing2_Peptone
ing3_Agar__if_needed_["Agar (if needed)"]
CultureMech_008342 --> ing3_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing3_Agar__if_needed_ -.-> CHEBI_2509
ing4_Seawater["Seawater"]
CultureMech_008342 --> ing4_Seawater
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Starch | 10 G_PER_L |
CHEBI:28017
starch
|
TOGO M1776 / NBRC Medium 992 lists 10 g/L starch. |
| 2 | Yeast extract | 4 G_PER_L |
FOODON:03315426
yeast extract
|
TOGO M1776 / NBRC Medium 992 lists 4 g/L yeast extract. |
| 3 | Peptone | 2 G_PER_L |
MICRO:0000178 (source link unavailable)
peptone
|
TOGO M1776 / NBRC Medium 992 lists 2 g/L generic peptone. |
| 4 | Agar (if needed) | 18 G_PER_L |
CHEBI:2509
agar
|
TOGO M1776 / NBRC Medium 992 lists 18 g/L agar if needed. |
| 5 | Seawater | 1000 ML_PER_L | NBRC Medium 992 lists 1 L seawater and specifies filtered, aged seawater or artificial seawater such as Daigo's Artificial Seawater SP. |
Preparation
- Combine 10 g starch, 4 g yeast extract, 2 g peptone, 18 g agar if needed, and 1 L filtered aged or artificial seawater.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.334495Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1776 |
| 2026-01-28T19:48:14.374879Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:23.900486+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:55.889988Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008342 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.434889+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "M1 Medium" to "m1_medium" for consistent matching |
| 2026-05-16T09:03:39.442367+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:11.760677+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m1776_m1_score15.py | RESOLVED_TOGO_M1776_M1_SCORE15 | TOGO M1776 imports NBRC Medium 992 M1 Medium. NBRC 992 lists 10 g starch, 4 g yeast extract, 2 g peptone, 18 g agar if needed, and 1 L seawater, with seawater supplied as filtered aged seawater or artificial seawater such as Daigo's Artificial Seawater SP. Moved the imported Seawater* placeholder solution into the main recipe as 1000 ml/L seawater, grounded the other four ingredients, and retained seawater as the one intentionally unmapped complex component. |