leeming_amp_notman_agar_modified_mlna

CultureMech:008353 bacterial COMPLEX SOLID_AGAR
Original name: Leeming & Notman agar Modified (MLNA)

Overview

External ontology
TOGO:M1786 — Leeming & Notman agar Modified (MLNA)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1786 Original source: NBRC - NBRC_M1004 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1004

Composition

flowchart LR
    CultureMech_008353["leeming_amp_notman_agar_modified_mlna"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008353 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008353 --> ing1_Yeast_extract
    ing2_Glycerol["Glycerol"]
    CultureMech_008353 --> ing2_Glycerol
    CHEBI_17754["CHEBI:17754"]
    ing2_Glycerol -.-> CHEBI_17754
    ing3_Olive_oil["Olive oil"]
    CultureMech_008353 --> ing3_Olive_oil
    ing4_Ox_bile__desiccated["Ox bile, desiccated"]
    CultureMech_008353 --> ing4_Ox_bile__desiccated
    ing5_Glucose["Glucose"]
    CultureMech_008353 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Agar["Agar"]
    CultureMech_008353 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509
    ing7_Glycerol_monostearate["Glycerol monostearate"]
    CultureMech_008353 --> ing7_Glycerol_monostearate
    CHEBI_75456["CHEBI:75456"]
    ing7_Glycerol_monostearate -.-> CHEBI_75456
    ing8_Tween_60["Tween 60"]
    CultureMech_008353 --> ing8_Tween_60
    CHEBI_53425["CHEBI:53425"]
    ing8_Tween_60 -.-> CHEBI_53425
    ing9_Bacteriological_peptone["Bacteriological peptone"]
    CultureMech_008353 --> ing9_Bacteriological_peptone

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
965 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 Glycerol
C3H8O3 92.094 g/mol
10 G_PER_L CHEBI:17754
glycerol
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
4 Olive oil 20 G_PER_L Role: Carbon source; Properties: Undefined component
5 Ox bile, desiccated 8 G_PER_L Role: Selective agent; Properties: Undefined component
6 Glucose
C6H12O6 180.156 g/mol
10 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
7 Agar 15 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
8 Glycerol monostearate
C21H42O4 358.563 g/mol
0.5 G_PER_L CHEBI:75456
2-stearoylglycerol
 Properties: Defined component, Organic compound, Simple component
9 Tween 60
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H46O6 606.838 g/mol
5 G_PER_L CHEBI:53425
polysorbate 60
 Properties: Defined component, Organic compound, Simple component
10 Bacteriological peptone 10 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.354401Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1786
2026-01-28T19:48:24.884374Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:55.987086Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008353
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.542749+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Leeming & Notman agar Modified (MLNA)" to "leeming_amp_notman_agar_modified_mlna" for consistent matching
2026-06-05T07:41:11.044924+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:40.343312+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).