archaeoglobus_fulgidus_medium

CultureMech:008362 archaea COMPLEX LIQUID
Original name: Archaeoglobus fulgidus Medium

Overview

External ontology
TOGO:M1794 — Archaeoglobus fulgidus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1794 Original source: NBRC - NBRC_M1019 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1019

Composition

flowchart LR
    CultureMech_008362["archaeoglobus_fulgidus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008362 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_008362 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008362 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_008362 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_Resazurin["Resazurin"]
    CultureMech_008362 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_008362 --> ing5_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_6H2O -.-> CHEBI_86345
    ing6_Na2S_9H2O["Na2S·9H2O"]
    CultureMech_008362 --> ing6_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing6_Na2S_9H2O -.-> CHEBI_76209
    ing7_Na2CO3["Na2CO3"]
    CultureMech_008362 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_Na2SO4["Na2SO4"]
    CultureMech_008362 --> ing8_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing8_Na2SO4 -.-> CHEBI_32149
    ing9_Sodium_lactate["Sodium lactate"]
    CultureMech_008362 --> ing9_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing9_Sodium_lactate -.-> CHEBI_75228
    ing10_Fe_NH4_2_SO4_2_7H2O["Fe(NH4)2(SO4)2·7H2O"]
    CultureMech_008362 --> ing10_Fe_NH4_2_SO4_2_7H2O
    CHEBI_131378["CHEBI:131378"]
    ing10_Fe_NH4_2_SO4_2_7H2O -.-> CHEBI_131378
    ing11_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_008362 --> ing11_Bacto_Yeast_Extract__Difco_
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_008362 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_008362 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_KH2PO4["KH2PO4"]
    CultureMech_008362 --> ing14_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing14_KH2PO4 -.-> CHEBI_63036
    ing15_K2HPO4["K2HPO4"]
    CultureMech_008362 --> ing15_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing15_K2HPO4 -.-> CHEBI_131527
    ing16_N2["N2"]
    CultureMech_008362 --> ing16_N2
    ing16_N2 -.-> CHEBI_17997
    ing17_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008362 --> ing17_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing17_Na2MoO4_2H2O -.-> CHEBI_75213
    ing18_H3BO3["H3BO3"]
    CultureMech_008362 --> ing18_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing18_H3BO3 -.-> CHEBI_33118
    ing19_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008362 --> ing19_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing19_MnCl2_4H2O -.-> CHEBI_86368
    ing20_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008362 --> ing20_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing20_CoCl2_6H2O -.-> CHEBI_35696
    ing21_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008362 --> ing21_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing21_NiCl2_6H2O -.-> CHEBI_34887
    ing22_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008362 --> ing22_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing22_CuCl2_2H2O -.-> CHEBI_86318
    ing23_ZnCl2["ZnCl2"]
    CultureMech_008362 --> ing23_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing23_ZnCl2 -.-> CHEBI_49976
    ing24_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008362 --> ing24_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing24_FeCl3_6H2O -.-> CHEBI_86254
    ing25_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
    CultureMech_008362 --> ing25_Nitrilotriacetic_acid__NTA_
    CHEBI_44557["CHEBI:44557"]
    ing25_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
    ing26_Na2WO4["Na2WO4"]
    CultureMech_008362 --> ing26_Na2WO4
    CHEBI_63940["CHEBI:63940"]
    ing26_Na2WO4 -.-> CHEBI_63940
    ing27_Na2SeO4["Na2SeO4"]
    CultureMech_008362 --> ing27_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing27_Na2SeO4 -.-> CHEBI_77775
    ing28_NaOH["NaOH"]
    CultureMech_008362 --> ing28_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing28_NaOH -.-> CHEBI_32145
    ing29_Biotin["Biotin"]
    CultureMech_008362 --> ing29_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing29_Biotin -.-> CHEBI_15956
    CultureMech_008362 --> more["...8 more"]

Ingredients (38)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 4 identical duplicates: 1.0, 1.0, 1.0, 1.0]
2 NaCl
Cl.Na 58.443 g/mol
31.0 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 30.0, 1.0]
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.15, 0.1]
4 NH4Cl
Cl.H4N 53.492 g/mol
0.54 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
6 MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
0.75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
0.36 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
8 Na2CO3
CO3.2Na 105.988 g/mol
1 G_PER_L CHEBI:29377
sodium carbonate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
9 Na2SO4
2Na.O4S 142.043 g/mol
2.4 G_PER_L CHEBI:32149
sodium sulfate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 Sodium lactate
C3H5O3.Na 112.06 g/mol
2.2 G_PER_L CHEBI:75228
sodium lactate
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
11 Fe(NH4)2(SO4)2·7H2O
Fe.7H2O.2H4N.2O4S 410.154 g/mol
2 G_PER_L CHEBI:131378
ferrous ammonium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 Bacto Yeast Extract (Difco) 0.5 G_PER_L Properties: Undefined component
13 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
Properties: Gas
14 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas
15 KH2PO4
H2O4P.K 136.084 g/mol
119 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
16 K2HPO4
HO4P.2K 174.174 g/mol
21 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
17 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
Properties: Gas
18 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21 CoCl2·6H2O
2Cl.Co 129.839 g/mol
0.024 G_PER_L CHEBI:35696
cobalt dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22 NiCl2·6H2O
Cl2Ni 129.599 g/mol
0.12 G_PER_L CHEBI:34887
nickel dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
23 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
0.025 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24 ZnCl2
2Cl.Zn 136.296 g/mol
0.1 G_PER_L CHEBI:49976
zinc dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
1.35 G_PER_L CHEBI:86254
iron trichloride hexahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26 Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
12.8 G_PER_L CHEBI:44557
nitrilotriacetic acid
Role: Mineral source; Properties: Organic compound, Defined component, Simple component
27 Na2WO4
2Na.O4W 293.816 g/mol
0.004 G_PER_L CHEBI:63940
sodium tungstate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
28 Na2SeO4
2Na.O4Se 188.936 g/mol
0.004 G_PER_L CHEBI:77775
sodium selenate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
29 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
Properties: Defined component, Inorganic compound, Solution
30 Biotin
C10H16N2O3S 244.316 g/mol
2 G_PER_L CHEBI:15956
biotin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
31 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
1 G_PER_L CHEBI:30753
4-aminobenzoic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
32 Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
5 G_PER_L CHEBI:49105
thiamine hydrochloride
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33 Ca-pantothenate
C9H16NO5 218.229 g/mol
5 G_PER_L CHEBI:29032
(R)-pantothenate
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34 Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
10 G_PER_L CHEBI:30961
pyridoxine hydrochloride
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
35 Folic acid
C19H19N7O6 441.404 g/mol
2 G_PER_L CHEBI:27470
folic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
36 Vitamin B12 0.01 G_PER_L CHEBI:176843
vitamin B12
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
37 Riboflavin
C17H20N4O6 376.369 g/mol
5 G_PER_L CHEBI:17015
riboflavin
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
38 Nicotinic acid
C6H5NO2 123.111 g/mol
5 G_PER_L CHEBI:15940
nicotinic acid
Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

KP buffer*

Dose: 5 G_PER_L

Trace element solution**

Dose: 2 G_PER_L

See mediadive_6187_Trace_element_solution.yaml for composition

Vitamin solution***

Dose: 2 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.376513Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1794
2026-01-28T19:48:30.236998Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2; Added default concentration for ingredient: NaOH
2026-03-09T08:04:50.253547+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T03:09:21.461458Z data-quality-cleanup-v1.0 Data quality fixes Merged 5 duplicate ingredient(s)
2026-03-13T06:19:56.102419Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008362
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:29.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.662355+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Archaeoglobus fulgidus Medium" to "archaeoglobus_fulgidus_medium" for consistent matching
2026-05-16T09:03:11.269737+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:34.382537+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:31.049162+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:48.208417+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 8 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-02T07:37:26.448112+00:00 repair_merged_duplicates.py REPAIRED_SUMMED_DUPLICATE_MERGE Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched.