Overview
- External ontology
- TOGO:M1799 — Alkali B medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1799 Original source: NBRC - NBRC_M1025 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1025
Composition
flowchart LR
CultureMech_008367["alkali_b_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008367 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008367 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008367 --> ing2_Yeast_extract
ing3_K2HPO4["K2HPO4"]
CultureMech_008367 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008367 --> ing4_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing4_CuSO4_5H2O -.-> CHEBI_31440
ing5_Glucose["Glucose"]
CultureMech_008367 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_L_Asparagine["L-Asparagine"]
CultureMech_008367 --> ing6_L_Asparagine
CHEBI_17196["CHEBI:17196"]
ing6_L_Asparagine -.-> CHEBI_17196
ing7_Agar["Agar"]
CultureMech_008367 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Fe2_SO4_3_6H2O["Fe2(SO4)3·6H2O"]
CultureMech_008367 --> ing8_Fe2_SO4_3_6H2O
ing9_Peptone["Peptone"]
CultureMech_008367 --> ing9_Peptone
ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008367 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
ing11_Na2CO3["Na2CO3"]
CultureMech_008367 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
ing12_MnSO4_7H2O["MnSO4·7H2O"]
CultureMech_008367 --> ing12_MnSO4_7H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_7H2O -.-> CHEBI_86360
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
500.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 500.0, 500.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.001 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 7 |
L-Asparagine
C4H8N2O3 132.119 g/mol
|
0.3 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Fe2(SO4)3·6H2O | 0.01 G_PER_L | Role: Mineral source | |
| 10 | Peptone | 0.2 G_PER_L | ||
| 11 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.001 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
10 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 13 |
MnSO4·7H2O
Mn.O4S 151.001 g/mol
|
0.001 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Solution A*
Dose: 500 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B**
Dose: 500 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.390787Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1799 |
| 2026-01-28T19:48:29.082470Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:48.221684+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:19.221219Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.158742Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008367 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.723071+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkali B medium" to "alkali_b_medium" for consistent matching |
| 2026-05-16T09:03:23.583568+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:47.799577+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.492628+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.766801+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:55.515961+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |