alkaline_yeast_extract_malt_extract_agar

CultureMech:008373 bacterial COMPLEX SOLID_AGAR
Original name: Alkaline yeast extract-malt extract agar

Overview

External ontology
TOGO:M1803 — Alkaline yeast extract-malt extract agar
Applications
  • Microbial cultivation
pH
10.0
Notes
NBRC Medium 1029 defines Alkaline yeast extract-malt extract agar with 4 g Bacto Yeast Extract (Difco), 10 g Bacto Malt Extract (Difco), 4 g glucose, 15 g agar, and 1 L artificial seawater, then adjusts the autoclaved medium to pH 10.0 with sterilized 10% Na2CO3 solution.

Composition

flowchart LR
    CultureMech_008373["alkaline_yeast_extract_malt_extract_agar"]
    ing0_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_008373 --> ing0_Bacto_Yeast_Extract__Difco_
    ing1_Bacto_Malt_Extract__Difco_["Bacto Malt Extract (Difco)"]
    CultureMech_008373 --> ing1_Bacto_Malt_Extract__Difco_
    ing2_Glucose["Glucose"]
    CultureMech_008373 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Agar["Agar"]
    CultureMech_008373 --> ing3_Agar
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar -.-> CHEBI_2509
    ing4_Artificial_seawater["Artificial seawater"]
    CultureMech_008373 --> ing4_Artificial_seawater

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Bacto Yeast Extract (Difco) 4.0 G_PER_L FOODON:03315426
yeast extract
 TOGO M1803 / NBRC Medium 1029 lists 4 g/L Bacto Yeast Extract (Difco).
2 Bacto Malt Extract (Difco) 10.0 G_PER_L FOODON:03301056
malt extract
 TOGO M1803 / NBRC Medium 1029 lists 10 g/L Bacto Malt Extract (Difco).
3 Glucose
C6H12O6 180.156 g/mol
4.0 G_PER_L CHEBI:17234
glucose
 TOGO M1803 / NBRC Medium 1029 lists 4.0 g/L Glucose.
4 Agar 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1803 / NBRC Medium 1029 lists 15.0 g/L Agar.
5 Artificial seawater 1000.0 ML_PER_L NBRC Medium 1029 lists 1 L artificial seawater per liter; the artificial seawater mixture is retained without a single-compound ontology grounding.

Preparation

  1. Mix Bacto Yeast Extract (Difco), Bacto Malt Extract (Difco), glucose, and agar in artificial seawater.
  2. Autoclave the basal medium.
  3. After autoclaving, adjust pH to 10.0 with sterilized 10% Na2CO3 solution.

Solutions

10% Na2CO3 solution

Dose: variable VARIABLE

Stock composition
IngredientConcentrationNotes
Na2CO3 10.0 PERCENT_W_V NBRC Medium 1029 specifies the pH-adjusting sodium carbonate solution as 10% w/v.
NBRC Medium 1029 uses sterilized 10% Na2CO3 solution to adjust the autoclaved medium to pH 10.0; the final addition volume is not specified.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.403852Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1803
2026-01-28T19:48:12.690191Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Na2CO3 solution
2026-03-09T08:04:20.968207+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:56.230446Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008373
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.795677+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Alkaline yeast extract-malt extract agar" to "alkaline_yeast_extract_malt_extract_agar" for consistent matching
2026-05-16T09:03:23.699188+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:47.945699+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1803_nbrc_1029_score15.py RESOLVED_TOGO_M1803_NBRC_1029 Verified TOGO M1803 against NBRC Medium 1029, corrected the imported artificial-seawater row from 1 g/L to 1000 ml/L, grounded the Bacto yeast extract, Bacto malt extract, glucose, agar, and 10% Na2CO3 solution, added pH 10.0, removed the stale MediaDive 7 match, and kept Artificial seawater as an intentionally unmapped input.