Overview
- External ontology
- TOGO:M1812 — Modified 1/5 SWS-Casein Starch Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1812 Original source: NBRC - NBRC_M1042 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1042
Composition
flowchart LR
CultureMech_008383["modified_1_5_sws_casein_starch_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008383 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008383 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008383 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008383 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008383 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_H3BO3["H3BO3"]
CultureMech_008383 --> ing5_H3BO3
CHEBI_33118["CHEBI:33118"]
ing5_H3BO3 -.-> CHEBI_33118
ing6_KCl["KCl"]
CultureMech_008383 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_NaHCO3["NaHCO3"]
CultureMech_008383 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_KBr["KBr"]
CultureMech_008383 --> ing8_KBr
CHEBI_32030["CHEBI:32030"]
ing8_KBr -.-> CHEBI_32030
ing9_Ferric_citrate["Ferric citrate"]
CultureMech_008383 --> ing9_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing9_Ferric_citrate -.-> CHEBI_144421
ing10_HEPES["HEPES"]
CultureMech_008383 --> ing10_HEPES
CHEBI_46756["CHEBI:46756"]
ing10_HEPES -.-> CHEBI_46756
ing11_SrCl2["SrCl2"]
CultureMech_008383 --> ing11_SrCl2
CHEBI_36383["CHEBI:36383"]
ing11_SrCl2 -.-> CHEBI_36383
ing12_Starch_from_corn__SIGMA_["Starch from corn (SIGMA)"]
CultureMech_008383 --> ing12_Starch_from_corn__SIGMA_
ing13_Casein_sodium["Casein sodium"]
CultureMech_008383 --> ing13_Casein_sodium
ing14___glycerophosphate_2Na["β-glycerophosphate-2Na"]
CultureMech_008383 --> ing14___glycerophosphate_2Na
ing15_Bacto_Agar__Difco_["Bacto Agar (Difco)"]
CultureMech_008383 --> ing15_Bacto_Agar__Difco_
ing16_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008383 --> ing16_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_2H2O -.-> CHEBI_75213
ing17_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008383 --> ing17_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_4H2O -.-> CHEBI_86368
ing18_ZnCl2["ZnCl2"]
CultureMech_008383 --> ing18_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing18_ZnCl2 -.-> CHEBI_49976
ing19_KI["KI"]
CultureMech_008383 --> ing19_KI
CHEBI_8346["CHEBI:8346"]
ing19_KI -.-> CHEBI_8346
ing20_CuSO4["CuSO4"]
CultureMech_008383 --> ing20_CuSO4
CHEBI_23414["CHEBI:23414"]
ing20_CuSO4 -.-> CHEBI_23414
ing21_CoCl2["CoCl2"]
CultureMech_008383 --> ing21_CoCl2
CHEBI_35696["CHEBI:35696"]
ing21_CoCl2 -.-> CHEBI_35696
ing22_SnCl2_2H2O["SnCl2·2H2O"]
CultureMech_008383 --> ing22_SnCl2_2H2O
CHEBI_78074["CHEBI:78074"]
ing22_SnCl2_2H2O -.-> CHEBI_78074
ing23_LiCl["LiCl"]
CultureMech_008383 --> ing23_LiCl
CHEBI_48607["CHEBI:48607"]
ing23_LiCl -.-> CHEBI_48607
ing24_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_008383 --> ing24_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing24_Fe_III__EDTA -.-> CHEBI_30729
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1.6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
H3BO3
H3BO3 61.833 g/mol
|
4 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KBr
Br.K 119.002 g/mol
|
16 G_PER_L |
CHEBI:32030
potassium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.02 G_PER_L |
CHEBI:144421
iron(III) citrate
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 11 | HEPES | 2 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 12 |
SrCl2
2Cl.Sr 158.526 g/mol
|
6 G_PER_L |
CHEBI:36383
strontium dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 | Starch from corn (SIGMA) | 2 G_PER_L | Role: Carbon source; Properties: Organic compound, Defined component, Complex component | |
| 14 | Casein sodium | 0.5 G_PER_L | Role: Nitrogen source; Properties: Undefined component | |
| 15 | β-glycerophosphate-2Na | 2 G_PER_L | Role: Mineral source; Properties: Organic compound, Defined component, Simple component | |
| 16 | Bacto Agar (Difco) | 15 G_PER_L | Role: Solidifying component | |
| 17 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
10 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
20 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
KI
I.K 166.002 g/mol
|
20 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuSO4
Cu.O4S 159.609 g/mol
|
10 G_PER_L |
CHEBI:23414
copper(II) sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CoCl2
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
SnCl2·2H2O
2Cl.2H2O.Sn 225.647 g/mol
|
5 G_PER_L |
CHEBI:78074
tin(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
LiCl
Cl.Li 42.394 g/mol
|
5 G_PER_L |
CHEBI:48607
lithium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
8 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Cyanocobalamin**
Dose: 0.5 G_PER_L
Role: Vitamin; Properties: Organic compound, Defined component, Simple component
Trace element solution*
Dose: 0.2 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.421401Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1812 |
| 2026-01-28T19:48:31.465703Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:52.445983+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:23.860043Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.333694Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008383 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.898557+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified 1/5 SWS-Casein Starch Medium" to "modified_1_5_sws_casein_starch_medium" for consistent matching |
| 2026-05-16T09:04:24.906405+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:36.017047+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:25.555605+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.453538+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:51.276921+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |