1_5_tsb_ph_6_5

CultureMech:008396 bacterial COMPLEX SOLID_AGAR
Original name: 1/5 TSB (pH 6.5)

Overview

External ontology
TOGO:M1824 — 1/5 TSB (pH 6.5)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1824 Original source: NBRC - NBRC_M1057 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1057 Commercial Product: Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Supplier: Multiple suppliers (BD, Sigma, Neogen) Catalog: Multiple (22092 Sigma, 211825 BD) Source: https://en.wikipedia.org/wiki/Tryptic_soy_broth Accessed: 2026-03-28 Constituent composition researched from product specification.

Composition

flowchart LR
    CultureMech_008396["1_5_tsb_ph_6_5"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008396 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008396 --> ing1_Agar__if_needed_
    ing2_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
    CultureMech_008396 --> ing2_Pancreatic_digest_of_casein
    ing3_Peptic_digest_of_soybean_meal["Peptic digest of soybean meal"]
    CultureMech_008396 --> ing3_Peptic_digest_of_soybean_meal
    ing4_Glucose["Glucose"]
    CultureMech_008396 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Sodium_chloride["Sodium chloride"]
    CultureMech_008396 --> ing5_Sodium_chloride
    CHEBI_26710["CHEBI:26710"]
    ing5_Sodium_chloride -.-> CHEBI_26710
    ing6_Dipotassium_phosphate["Dipotassium phosphate"]
    CultureMech_008396 --> ing6_Dipotassium_phosphate
    CHEBI_131527["CHEBI:131527"]
    ing6_Dipotassium_phosphate -.-> CHEBI_131527
    ing7_Agar["Agar"]
    CultureMech_008396 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Agar (if needed) 15 G_PER_L Role: Solidifying component
3 Pancreatic digest of casein
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
17.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 Pancreatic enzymatic digest of casein providing amino acids and peptides; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
4 Peptic digest of soybean meal
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
3.0 G_PER_L FOODON:03315720
vegetable protein, hydrolyzed
 Peptic digest of soybean meal providing additional peptides and carbohydrates; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
5 Glucose
C6H12O6 180.156 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:17234
glucose
 Carbon/energy source; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
6 Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
5.0 G_PER_L CHEBI:26710
sodium chloride
 Maintains osmotic equilibrium; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
7 Dipotassium phosphate
HO4P.2K 174.174 g/mol
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
2.5 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 pH buffer; Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product
8 Agar
Multiple suppliers (BD, Sigma, Neogen) Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) Multiple (22092 Sigma, 211825 BD)
15.0 G_PER_L CHEBI:2509
agar
 Solidifying agent (for TSA only, not in TSB); Constituent of Tryptic Soy Broth (TSB) / Tryptic Soy Agar (TSA) commercial product

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.444821Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1824
2026-01-28T19:48:24.535635Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:56.437859Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008396
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:15.005728+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 TSB (pH 6.5)" to "1_5_tsb_ph_6_5" for consistent matching
2026-06-05T07:40:15.662225+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.