Overview
- External ontology
- TOGO:M1829 — Pae I Medium + 0.1% (w/v) Urea
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1829 Original source: NBRC - NBRC_M1062 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1062
Composition
flowchart LR
CultureMech_008401["pae_i_medium_0_1_w_v_urea"]
ing0_Distilled_water["Distilled water"]
CultureMech_008401 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008401 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008401 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar__if_needed_["Agar (if needed)"]
CultureMech_008401 --> ing3_Agar__if_needed_
ing4_Tryptone["Tryptone"]
CultureMech_008401 --> ing4_Tryptone
ing5_Urea["Urea"]
CultureMech_008401 --> ing5_Urea
CHEBI_16199["CHEBI:16199"]
ing5_Urea -.-> CHEBI_16199
ing6_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_008401 --> ing6_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing6_Tris_HCl_buffer -.-> CHEBI_9754
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 3 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 5 | Tryptone | 5 G_PER_L | Properties: Undefined component | |
| 6 |
Urea
CH4N2O 60.056 g/mol
|
1 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Simple component, Organic compound |
| 7 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
variable VARIABLE |
CHEBI:9754
tris
|
Role: Buffer; Properties: Complex component, Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.452549Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1829 |
| 2026-01-28T19:48:19.501433Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Tris-HCl buffer |
| 2026-03-13T06:19:56.473855Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008401 |
| 2026-03-17T06:30:07.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.042392+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pae I Medium + 0.1% (w/v) Urea" to "pae_i_medium_0_1_w_v_urea" for consistent matching |
| 2026-05-16T09:04:28.336743+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.796291+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |