alb_medium

Overview

External ontology

TOGO:M1830 — ALB Medium

Applications

• Microbial cultivation

pH range

{'min': 8.0, 'max': 9.0, 'notes': 'NBRC Medium 1063 lists pH 8-9.'}

Notes

TOGO M1830 imports NBRC Medium 1063 ALB Medium: 10.0 g/L tryptone, 5.0 g/L yeast extract, 5.0 g/L NaCl, 2.0 g/L separately autoclaved Na2CO3, 1000.0 ml/L distilled water, and 15.0 g/L agar if needed, with final pH 8-9 under air containing 5% CO2.

Composition

flowchart LR
    CultureMech_008403["alb_medium"]
    ing0_Tryptone["Tryptone"]
    CultureMech_008403 --> ing0_Tryptone
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008403 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008403 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Na2CO3["Na2CO3"]
    CultureMech_008403 --> ing3_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing3_Na2CO3 -.-> CHEBI_29377
    ing4_Distilled_water["Distilled water"]
    CultureMech_008403 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008403 --> ing5_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (6)

Preparation

1. Dissolve 10.0 g/L tryptone, 5.0 g/L yeast extract, 5.0 g/L NaCl, and 15.0 g/L agar if needed in 1000.0 ml/L distilled water.
2. Dissolve 2.0 g/L Na2CO3 separately.
3. Adjust the final ALB Medium to pH 8-9.
4. Autoclave the basal medium and the Na2CO3 solution separately.
5. Aseptically add sterile Na2CO3 to the autoclaved basal medium.
6. Use ALB Medium under air containing 5% CO2.

Target organisms

No target organisms are recorded.

Provenance