lmg_157_medium

CultureMech:008429 bacterial COMPLEX SOLID_AGAR
Original name: LMG 157 medium

Overview

External ontology
TOGO:M1854 — LMG 157 medium
Applications
  • Microbial cultivation
pH range
{'min': 7.2, 'max': 7.4}
Notes
TOGO M1854 imports NBRC Medium 1095 LMG 157 medium. NBRC 1095 lists 0.5 g/L Beef extract, 0.5 g/L Yeast extract, 2 g/L Tryptone, 0.2 g/L Sodium acetate, 0.25 g/L Glucose sterilized separately by autoclaving, 1000 ml/L Distilled water, 15 g/L agar if needed, and pH 7.2-7.4.

Composition

flowchart LR
    CultureMech_008429["lmg_157_medium"]
    ing0_Beef_extract["Beef extract"]
    CultureMech_008429 --> ing0_Beef_extract
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008429 --> ing1_Yeast_extract
    ing2_Tryptone["Tryptone"]
    CultureMech_008429 --> ing2_Tryptone
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_008429 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_Glucose["Glucose"]
    CultureMech_008429 --> ing4_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing4_Glucose -.-> CHEBI_17234
    ing5_Distilled_water["Distilled water"]
    CultureMech_008429 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008429 --> ing6_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Beef extract 0.5 G_PER_L NBRC Medium 1095 lists 0.5 g/L Beef extract as a complex meat extract not reducible to one ChEBI molecule.
2 Yeast extract 0.5 G_PER_L FOODON:03315426
yeast extract
 TOGO M1854 / NBRC Medium 1095 lists 0.5 g/L Yeast extract.
3 Tryptone 2 G_PER_L NBRC Medium 1095 lists 2 g/L Tryptone as a digest product not reducible to one ChEBI molecule.
4 Sodium acetate
C2H3O2.Na 82.034 g/mol
0.2 G_PER_L CHEBI:32954
sodium acetate
 TOGO M1854 / NBRC Medium 1095 lists 0.2 g/L Sodium acetate.
5 Glucose
C6H12O6 180.156 g/mol
0.25 G_PER_L CHEBI:17234
glucose
 NBRC Medium 1095 lists 0.25 g/L Glucose, separately autoclaved and added to the other ingredients for a final concentration of 0.025%.
6 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 TOGO M1854 / NBRC Medium 1095 lists 1000 ml/L Distilled water.
7 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 TOGO M1854 / NBRC Medium 1095 lists 15 g/L agar if needed.

Preparation

  1. Prepare the base from 0.5 g/L beef extract, 0.5 g/L yeast extract, 2 g/L tryptone, 0.2 g/L sodium acetate, 1000 ml/L distilled water, and 15 g/L agar if needed.
  2. Sterilize 0.25 g/L glucose separately by autoclaving.
  3. Add the separately autoclaved glucose to the other ingredients for a final glucose concentration of 0.025%.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.516894Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1854
2026-01-28T19:48:42.752001Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:05:12.056672+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:56.785185Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008429
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:56.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:15.357685+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LMG 157 medium" to "lmg_157_medium" for consistent matching
2026-05-16T09:03:39.190769+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:11.376801+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1854_lmg157_score15.py RESOLVED_TOGO_M1854_LMG157_SCORE15 TOGO M1854 imports NBRC Medium 1095 LMG 157 medium. NBRC 1095 lists 0.5 g/L Beef extract, 0.5 g/L Yeast extract, 2 g/L Tryptone, 0.2 g/L Sodium acetate, 0.25 g/L Glucose sterilized separately by autoclaving, 1000 ml/L Distilled water, 15 g/L agar if needed, and pH 7.2-7.4. Corrected the imported distilled-water row from a g/L artifact to 1000 ml/L and moved the Glucose* placeholder solution back into the main composition as 0.25 g/L glucose with separate autoclaving captured in preparation steps.