Overview
- External ontology
- TOGO:M1859 — Medium for strain R-1
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1859 Original source: NBRC - NBRC_M1103 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1103
Composition
flowchart LR
CultureMech_008433["medium_for_strain_r_1"]
ing0_Distilled_water["Distilled water"]
CultureMech_008433 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008433 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008433 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008433 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008433 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_NH4Cl["NH4Cl"]
CultureMech_008433 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_008433 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Biotin["Biotin"]
CultureMech_008433 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008433 --> ing8_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing8_Thiamine_HCl -.-> CHEBI_49105
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_008433 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008433 --> ing10_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing10_FeCl3_6H2O -.-> CHEBI_86254
ing11_Sodium_succinate["Sodium succinate"]
CultureMech_008433 --> ing11_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing11_Sodium_succinate -.-> CHEBI_63675
ing12_Sodium_propionate["Sodium propionate"]
CultureMech_008433 --> ing12_Sodium_propionate
CHEBI_132106["CHEBI:132106"]
ing12_Sodium_propionate -.-> CHEBI_132106
ing13_Agar__if_needed_["Agar (if needed)"]
CultureMech_008433 --> ing13_Agar__if_needed_
ing14_Sodium_malate["Sodium malate"]
CultureMech_008433 --> ing14_Sodium_malate
CHEBI_91260["CHEBI:91260"]
ing14_Sodium_malate -.-> CHEBI_91260
ing15_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008433 --> ing15_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing15_Na2S_9H2O -.-> CHEBI_76209
ing16_H2SO4["H2SO4"]
CultureMech_008433 --> ing16_H2SO4
CHEBI_26836["CHEBI:26836"]
ing16_H2SO4 -.-> CHEBI_26836
ing17_N2["N2"]
CultureMech_008433 --> ing17_N2
CHEBI_17997["CHEBI:17997"]
ing17_N2 -.-> CHEBI_17997
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.4 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.01 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.2 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 11 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.005 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.5 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 13 |
Sodium propionate
C3H5O2.Na 96.061 g/mol
|
0.5 G_PER_L |
CHEBI:132106
sodium propionate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 14 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 15 | Sodium malate | 0.5 G_PER_L |
CHEBI:91260
disodium malate
|
Properties: Defined component, Organic compound, Simple component |
| 16 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
1.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 18 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.525694Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1859 |
| 2026-01-28T19:48:06.708543Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-13T03:08:37.367873Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.828526Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008433 |
| 2026-03-17T06:29:38.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.401655+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for strain R-1" to "medium_for_strain_r_1" for consistent matching |
| 2026-05-16T09:04:20.623632+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:29.751614+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:21.545034+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.306241+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |