Overview
- External ontology
- TOGO:M1862 — 1a Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1862 Original source: NBRC - NBRC_M1106 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1106
Composition
flowchart LR
CultureMech_008437["1a_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008437 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008437 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008437 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008437 --> ing3_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_7H2O -.-> CHEBI_75836
ing4_NaNO3["NaNO3"]
CultureMech_008437 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Na2HPO4_2H2O["Na2HPO4·2H2O"]
CultureMech_008437 --> ing5_Na2HPO4_2H2O
CHEBI_34683["CHEBI:34683"]
ing5_Na2HPO4_2H2O -.-> CHEBI_34683
ing6_CaCl2_6H2O["CaCl2·6H2O"]
CultureMech_008437 --> ing6_CaCl2_6H2O
CHEBI_91243["CHEBI:91243"]
ing6_CaCl2_6H2O -.-> CHEBI_91243
ing7_Methane_gas["Methane gas"]
CultureMech_008437 --> ing7_Methane_gas
ing8_Air["Air"]
CultureMech_008437 --> ing8_Air
ing9_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008437 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_008437 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008437 --> ing11_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing11_CoCl2_6H2O -.-> CHEBI_35696
ing12_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008437 --> ing12_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing12_ZnSO4_7H2O -.-> CHEBI_32312
ing13_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008437 --> ing13_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing13_CuSO4_5H2O -.-> CHEBI_31440
ing14_MnSO4_2H2O["MnSO4·2H2O"]
CultureMech_008437 --> ing14_MnSO4_2H2O
CHEBI_86356["CHEBI:86356"]
ing14_MnSO4_2H2O -.-> CHEBI_86356
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.22 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2HPO4·2H2O
HO4P.2Na 141.958 g/mol
|
0.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
CaCl2·6H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.03 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 | Methane gas | variable VARIABLE | Properties: ガス | |
| 9 | Air | variable VARIABLE | Properties: ガス | |
| 10 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.06 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
0.1 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.08 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.2 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
MnSO4·2H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.17 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Trace metal solution*
Dose: 1 G_PER_L
See mediadive_6140_Trace_metal_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.532666Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1862 |
| 2026-01-28T19:48:30.474960Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:50.669097+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:21.936014Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.864295Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008437 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.439123+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "1a Medium" to "1a_medium" for consistent matching |
| 2026-05-16T09:02:58.196812+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:15.686292+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:12.443931+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:42.519531+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:37:32.626651+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |