Overview
- External ontology
- TOGO:M1866 — Alkalic MRS (pH 8.5) + 3% NaCl
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1866 Original source: NBRC - NBRC_M1110 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1110
Composition
flowchart LR
CultureMech_008441["alkalic_mrs_ph_8_5_3_nacl"]
ing0_Distilled_water["Distilled water"]
CultureMech_008441 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008441 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008441 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008441 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO4["K2HPO4"]
CultureMech_008441 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Sodium_acetate["Sodium acetate"]
CultureMech_008441 --> ing5_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing5_Sodium_acetate -.-> CHEBI_32954
ing6_Tween_80["Tween 80"]
CultureMech_008441 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_MnSO4_nH2O["MnSO4·nH2O"]
CultureMech_008441 --> ing7_MnSO4_nH2O
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4_nH2O -.-> CHEBI_86360
ing8_Diammonium_hydrogen_citrate["Diammonium hydrogen citrate"]
CultureMech_008441 --> ing8_Diammonium_hydrogen_citrate
CHEBI_63076["CHEBI:63076"]
ing8_Diammonium_hydrogen_citrate -.-> CHEBI_63076
ing9_Glucose["Glucose"]
CultureMech_008441 --> ing9_Glucose
CHEBI_17234["CHEBI:17234"]
ing9_Glucose -.-> CHEBI_17234
ing10_Agar__if_needed_["Agar (if needed)"]
CultureMech_008441 --> ing10_Agar__if_needed_
ing11_Meat_extract["Meat extract"]
CultureMech_008441 --> ing11_Meat_extract
ing12_Peptone["Peptone"]
CultureMech_008441 --> ing12_Peptone
ing13_Na2CO3["Na2CO3"]
CultureMech_008441 --> ing13_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing13_Na2CO3 -.-> CHEBI_29377
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
5 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
1 G_PER_L |
CHEBI:53426
polysorbate 80
|
Role: Surfactant; Properties: Defined component, Organic compound, Simple component |
| 8 |
MnSO4·nH2O
Mn.O4S 151.001 g/mol
|
0.05 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Diammonium hydrogen citrate
C6H6O7.2H4N 226.185 g/mol
|
2 G_PER_L |
CHEBI:63076
diammonium citrate
|
Role: Nutrient source; Properties: Defined component, Organic compound, Simple component |
| 10 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 11 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 12 | Meat extract | 10 G_PER_L | Properties: Undefined component | |
| 13 | Peptone | 10 G_PER_L | ||
| 14 |
Na2CO3
CO3.2Na 105.988 g/mol
|
6 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
Solutions
Solution A*
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B**
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.544061Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1866 |
| 2026-01-28T19:48:40.478095Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:08.233107+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:39.969632Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.914842Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008441 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.494910+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkalic MRS (pH 8.5) + 3% NaCl" to "alkalic_mrs_ph_8_5_3_nacl" for consistent matching |
| 2026-05-16T09:03:23.602893+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:47.827119+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:35.517168+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:52.776944+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |