Overview
- External ontology
- TOGO:M186 — Mmys-I Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M186 Original source: JCM - JCM_M193 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=193
Composition
flowchart LR
CultureMech_008444["mmys_i_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008444 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008444 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008444 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008444 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008444 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008444 --> ing5_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_6H2O -.-> CHEBI_86345
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_008444 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_Na2S2O3_5H2O["Na2S2O3・5H2O"]
CultureMech_008444 --> ing7_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing7_Na2S2O3_5H2O -.-> CHEBI_32150
ing8_Sodium_DL_malate["Sodium DL-malate"]
CultureMech_008444 --> ing8_Sodium_DL_malate
CHEBI_91260["CHEBI:91260"]
ing8_Sodium_DL_malate -.-> CHEBI_91260
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
30.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
Na2S2O3・5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 9 | Sodium DL-malate | 3.6 G_PER_L |
CHEBI:91260
disodium malate
|
Properties: Defined component, Simple component, Organic compound |
Solutions
Trace element solution SL8 (see Medium [M183])
Dose: 1 G_PER_L
Cross-reference to Medium M183
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.573853Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M186 |
| 2026-01-28T19:48:20.314031Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:33.666879+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:56.930192Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008444 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.513030+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mmys-I Medium" to "mmys_i_medium" for consistent matching |
| 2026-05-16T09:03:11.530557+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:34.660229+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.313057+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |