Overview
- External ontology
- TOGO:M1871 — Nitrate mineral salts medium (NMS) + 0.01% Tryptone
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1871 Original source: NBRC - NBRC_M1116 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1116
Composition
flowchart LR
CultureMech_008446["nitrate_mineral_salts_medium_nms_0_01_tryptone"]
ing0_Distilled_deionized_water["Distilled deionized water"]
CultureMech_008446 --> ing0_Distilled_deionized_water
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008446 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008446 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_KNO3["KNO3"]
CultureMech_008446 --> ing3_KNO3
CHEBI_63043["CHEBI:63043"]
ing3_KNO3 -.-> CHEBI_63043
ing4_Na2HPO4_12H2O["Na2HPO4·12H2O"]
CultureMech_008446 --> ing4_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing4_Na2HPO4_12H2O -.-> CHEBI_34683
ing5_CaCl2_6H2O["CaCl2·6H2O"]
CultureMech_008446 --> ing5_CaCl2_6H2O
CHEBI_91243["CHEBI:91243"]
ing5_CaCl2_6H2O -.-> CHEBI_91243
ing6_Purified_Agar__e_g___Oxoid_L28_["Purified Agar (e.g., Oxoid L28)"]
CultureMech_008446 --> ing6_Purified_Agar__e_g___Oxoid_L28_
ing7_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008446 --> ing7_Bacto_Tryptone__Difco_
ing8_Methane_gas["Methane gas"]
CultureMech_008446 --> ing8_Methane_gas
ing9_Air["Air"]
CultureMech_008446 --> ing9_Air
ing10_Distilled_water["Distilled water"]
CultureMech_008446 --> ing10_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing10_Distilled_water -.-> CHEBI_15377
ing11_HCl__concentrated_["HCl (concentrated)"]
CultureMech_008446 --> ing11_HCl__concentrated_
ing12_EDTA__sodium_salt["EDTA, sodium salt"]
CultureMech_008446 --> ing12_EDTA__sodium_salt
ing13_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008446 --> ing13_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing13_CaCl2_2H2O -.-> CHEBI_86158
ing14_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008446 --> ing14_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_2H2O -.-> CHEBI_75213
ing15_H3BO3["H3BO3"]
CultureMech_008446 --> ing15_H3BO3
CHEBI_33118["CHEBI:33118"]
ing15_H3BO3 -.-> CHEBI_33118
ing16_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008446 --> ing16_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_7H2O -.-> CHEBI_75836
ing17_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008446 --> ing17_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_4H2O -.-> CHEBI_86368
ing18_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008446 --> ing18_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing18_CoCl2_6H2O -.-> CHEBI_35696
ing19_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008446 --> ing19_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_6H2O -.-> CHEBI_34887
ing20_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008446 --> ing20_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_7H2O -.-> CHEBI_32312
ing21_EDTA["EDTA"]
CultureMech_008446 --> ing21_EDTA
CHEBI_4735["CHEBI:4735"]
ing21_EDTA -.-> CHEBI_4735
Ingredients (22)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Distilled deionized water | 1 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.272 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KNO3
K.NO3 101.102 g/mol
|
1 G_PER_L |
CHEBI:63043
potassium nitrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2HPO4·12H2O
HO4P.2Na 141.958 g/mol
|
0.717 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
CaCl2·6H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.2 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 | Purified Agar (e.g., Oxoid L28) | 12.5 G_PER_L | Role: Solidifying component | |
| 8 | Bacto Tryptone (Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 9 | Methane gas | variable VARIABLE | Properties: ガス | |
| 10 | Air | variable VARIABLE | Properties: ガス | |
| 11 |
Distilled water
H2O 18.015 g/mol
|
101.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 100.0, 1.0] |
| 12 | HCl (concentrated) | 0.3 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 13 | EDTA, sodium salt | 0.2 G_PER_L | Properties: Defined component, Simple component | |
| 14 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
200 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
10 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
EDTA
C10H16N2O8 292.244 g/mol
|
500 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Simple component, Organic compound, Defined component |
Solutions
Chelated Iron Solution*
Dose: 2 G_PER_L
See mediadive_4790_Chelated_iron_solution.yaml for composition
Trace Element Solution**
Dose: 0.5 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Ferric ammonium citrate***
Dose: 0.1 G_PER_L
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.549139Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1871 |
| 2026-01-28T19:48:20.546616Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Methane gas; Added default concentration for ingredient: Air |
| 2026-03-09T08:04:34.029260+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:03.551388Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:56.944159Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008446 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:09.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.528479+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Nitrate mineral salts medium (NMS) + 0.01% Tryptone" to "nitrate_mineral_salts_medium_nms_0_01_tryptone" for consistent matching |
| 2026-05-16T09:04:27.596174+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:39.681590+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:28.148271+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.549518+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |