1_5_marine_agar_broth

CultureMech:008494 bacterial COMPLEX SOLID_AGAR
Original name: 1/5 Marine Agar/Broth

Overview

External ontology
TOGO:M1916 — 1/5 Marine Agar/Broth
Applications
  • Microbial cultivation
pH
7.6
Notes
Source: https://togomedium.org/medium/M1916 Original source: NBRC - NBRC_M1180 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1180

Composition

flowchart LR
    CultureMech_008494["1_5_marine_agar_broth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008494 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008494 --> ing1_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing1_Agar__if_needed_ -.-> CHEBI_2509
    ing2_Bacto_Marine_Broth_2216__Difco_["Bacto Marine Broth 2216 (Difco)"]
    CultureMech_008494 --> ing2_Bacto_Marine_Broth_2216__Difco_

Ingredients (3)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
200 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component; TOGO M1916 lists agar only as an optional solidifying agent.
3 Bacto Marine Broth 2216 (Difco) 7 G_PER_L Properties: Undefined component, Complex component

Solutions

Sea water*

Dose: 800 G_PER_L

Role: Solvating media; Properties: Undefined component, Complex component

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.662186Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1916
2026-01-28T19:48:09.938537Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:16.161098+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:57.495442Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008494
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.107210+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "1/5 Marine Agar/Broth" to "1_5_marine_agar_broth" for consistent matching
2026-05-16T09:02:58.158601+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:15.626435+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_marine_broth_dilutions_score15.py RESOLVED_TOGO_MARINE_BROTH_DILUTION_SCORE15 TOGO M1916 mirrors NBRC Medium 1180 and lists agar only as the optional solidifying agent. TOGO reports pH 7.6 and preserves the source footnote identifying Sea water as filtered aged seawater or Daigo artificial seawater.