Overview
- External ontology
- TOGO:M1922 — Azospirillum amazonense medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1922 Original source: NBRC - NBRC_M1186 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1186
Composition
flowchart LR
CultureMech_008501["azospirillum_amazonense_medium"]
ing0_Bromophenol_blue__0_5__in_0_2N_KOH_["Bromophenol blue (0.5% in 0.2N KOH)"]
CultureMech_008501 --> ing0_Bromophenol_blue__0_5__in_0_2N_KOH_
ing1_Distilled_water["Distilled water"]
CultureMech_008501 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008501 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008501 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008501 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_K2HPO4["K2HPO4"]
CultureMech_008501 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008501 --> ing6_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing6_Na2MoO4_2H2O -.-> CHEBI_75213
ing7_Sucrose["Sucrose"]
CultureMech_008501 --> ing7_Sucrose
CHEBI_17992["CHEBI:17992"]
ing7_Sucrose -.-> CHEBI_17992
ing8_FeCl3["FeCl3"]
CultureMech_008501 --> ing8_FeCl3
CHEBI_30808["CHEBI:30808"]
ing8_FeCl3 -.-> CHEBI_30808
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bromophenol blue (0.5% in 0.2N KOH) | 5 G_PER_L | Role: pH indicator; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.02 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
2 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
Sucrose
C12H22O11 342.297 g/mol
|
5 G_PER_L |
CHEBI:17992
sucrose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 9 |
FeCl3
Cl3Fe 162.204 g/mol
|
0.01 G_PER_L |
CHEBI:30808
iron trichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.671204Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1922 |
| 2026-01-28T19:48:20.873359Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:57.542918Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008501 |
| 2026-03-17T06:30:10.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.157798+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Azospirillum amazonense medium" to "azospirillum_amazonense_medium" for consistent matching |
| 2026-06-05T07:40:34.922166+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:24.987063+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.408254+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |