sphaerotilus_leptothrix_medium

CultureMech:008502 bacterial COMPLEX SOLID_AGAR
Original name: Sphaerotilus-Leptothrix Medium

Overview

External ontology
TOGO:M1923 — Sphaerotilus-Leptothrix Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1923 Original source: NBRC - NBRC_M1188 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1188

Composition

flowchart LR
    CultureMech_008502["sphaerotilus_leptothrix_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008502 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008502 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008502 --> ing2_Yeast_extract
    ing3_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008502 --> ing3_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing3_FeCl3_6H2O -.-> CHEBI_86254
    ing4_CaCl2["CaCl2"]
    CultureMech_008502 --> ing4_CaCl2
    CHEBI_3312["CHEBI:3312"]
    ing4_CaCl2 -.-> CHEBI_3312
    ing5_MnSO4_H2O["MnSO4·H2O"]
    CultureMech_008502 --> ing5_MnSO4_H2O
    CHEBI_86364["CHEBI:86364"]
    ing5_MnSO4_H2O -.-> CHEBI_86364
    ing6_Ferric_ammonium_citrate["Ferric ammonium citrate"]
    CultureMech_008502 --> ing6_Ferric_ammonium_citrate
    CHEBI_31604["CHEBI:31604"]
    ing6_Ferric_ammonium_citrate -.-> CHEBI_31604
    ing7_Agar["Agar"]
    CultureMech_008502 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509
    ing8_Peptone["Peptone"]
    CultureMech_008502 --> ing8_Peptone

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
0.01 G_PER_L CHEBI:86254
iron trichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaCl2
Ca.2Cl 110.984 g/mol
0.05 G_PER_L CHEBI:3312
calcium dichloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
6 MnSO4·H2O
H2O.Mn.O4S 169.016 g/mol
0.05 G_PER_L CHEBI:86364
manganese(II) sulfate monohydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
7 Ferric ammonium citrate 0.5 G_PER_L CHEBI:31604
ferric ammonium citrate
 Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
8 Agar 20 G_PER_L CHEBI:2509
agar
 Role: Solidifying component
9 Peptone 1.5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.672587Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1923
2026-01-28T19:48:29.104462Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:57.548485Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008502
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.164151+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sphaerotilus-Leptothrix Medium" to "sphaerotilus_leptothrix_medium" for consistent matching
2026-06-05T07:40:34.929093+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:24.994733+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.