Overview
- External ontology
- TOGO:M1929 — Modified W-medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1929 Original source: NBRC - NBRC_M1196 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1196
Composition
flowchart LR
CultureMech_008507["modified_w_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008507 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008507 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008507 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_H3BO4["H3BO4"]
CultureMech_008507 --> ing3_H3BO4
ing4_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008507 --> ing4_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing4_FeSO4_7H2O -.-> CHEBI_75836
ing5_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008507 --> ing5_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing5_ZnSO4_7H2O -.-> CHEBI_32312
ing6__NH4_2SO4["(NH4)2SO4"]
CultureMech_008507 --> ing6__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing6__NH4_2SO4 -.-> CHEBI_62946
ing7_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008507 --> ing7_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing7_CuSO4_5H2O -.-> CHEBI_31440
ing8_Concentrated_HCl["Concentrated HCl"]
CultureMech_008507 --> ing8_Concentrated_HCl
ing9_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_008507 --> ing9_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing9_CoSO4_7H2O -.-> CHEBI_91244
ing10_CaCO3["CaCO3"]
CultureMech_008507 --> ing10_CaCO3
CHEBI_3311["CHEBI:3311"]
ing10_CaCO3 -.-> CHEBI_3311
ing11_MnSO4_4H2O["MnSO4·4H2O"]
CultureMech_008507 --> ing11_MnSO4_4H2O
CHEBI_86358["CHEBI:86358"]
ing11_MnSO4_4H2O -.-> CHEBI_86358
ing12_Na2HPO4_7H2O["Na2HPO4·7H2O"]
CultureMech_008507 --> ing12_Na2HPO4_7H2O
CHEBI_34683["CHEBI:34683"]
ing12_Na2HPO4_7H2O -.-> CHEBI_34683
ing13_MgO["MgO"]
CultureMech_008507 --> ing13_MgO
ing14_Agar__if_needed_["Agar (if needed)"]
CultureMech_008507 --> ing14_Agar__if_needed_
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.85 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 | H3BO4 | 0.06 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 5 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
9.5 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
1.44 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.25 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 | Concentrated HCl | 51.3 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 10 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.28 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CaCO3
CO3.Ca 100.086 g/mol
|
2 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Mineral source, Protective agent; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnSO4·4H2O
4H2O.Mn.O4S 223.061 g/mol
|
1.12 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
Na2HPO4·7H2O
HO4P.2Na 141.958 g/mol
|
4.9 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 14 | MgO | 10.75 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 15 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component |
Solutions
Substrates*
Dose: 10 MILLIMOLAR
Role: Carbon source; Properties: Organic compound, Defined component, Simple component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.689000Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1929 |
| 2026-01-28T19:48:08.013040Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:12.882491+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:57.621237Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008507 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.239247+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified W-medium" to "modified_w_medium" for consistent matching |
| 2026-05-16T09:04:26.431484+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:38.016516+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.971998+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:09.032749+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |