plate_count_agar_pca

CultureMech:008509 bacterial COMPLEX SOLID_AGAR
Original name: Plate Count Agar (PCA)

Overview

External ontology
TOGO:M1930 — Plate Count Agar (PCA)
Applications
  • Microbial cultivation
pH
7.2
Notes
TOGO M1930 imports NBRC Medium 1197 Plate Count Agar (PCA). NBRC 1197 lists 5 g tryptone, 2.5 g yeast extract, 1 g glucose, 15 g agar if needed, 1 L water, and pH 7.2.

Composition

flowchart LR
    CultureMech_008509["plate_count_agar_pca"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008509 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008509 --> ing1_Yeast_extract
    ing2_Glucose["Glucose"]
    CultureMech_008509 --> ing2_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing2_Glucose -.-> CHEBI_17234
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008509 --> ing3_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing3_Agar__if_needed_ -.-> CHEBI_2509
    ing4_Tryptone["Tryptone"]
    CultureMech_008509 --> ing4_Tryptone

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 TOGO M1930 / NBRC Medium 1197 lists 1 L water as the solvent; the source label typo 'Disrilled water' is normalized to Distilled water.
2 Yeast extract 2.5 G_PER_L FOODON:03315426
Yeast extract
 TOGO M1930 / NBRC Medium 1197 lists 2.5 g/L Yeast extract.
3 Glucose
C6H12O6 180.156 g/mol
1.0 G_PER_L CHEBI:17234
glucose
 TOGO M1930 / NBRC Medium 1197 lists 1 g/L Glucose.
4 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1930 / NBRC Medium 1197 lists 15 g/L Agar if needed.
5 Tryptone 5.0 G_PER_L MICRO:0000182 (source link unavailable)
tryptone
 TOGO M1930 / NBRC Medium 1197 lists 5 g/L Tryptone.

Preparation

  1. Adjust pH to 7.2.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.691352Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1930
2026-01-28T19:48:24.479129Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:57.633513Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008509
2026-03-17T06:30:17.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.251763+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Plate Count Agar (PCA)" to "plate_count_agar_pca" for consistent matching
2026-06-05T07:41:42.241786+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1930_plate_count_agar_score15.py RESOLVED_TOGO_M1930_PLATE_COUNT_AGAR_SCORE15 Corrected NBRC 1197 water from an imported 1 g/L 'Disrilled water' row to 1 L distilled water, added pH 7.2, grounded every PCA component, and removed a stale MediaDive Medium 21 match.