Overview
- External ontology
- TOGO:M1943 — Halomarina medium
- Applications
-
- Microbial cultivation
- pH
- 7.5
- Notes
- NBRC Medium 1214 records Halomarina medium as 1 g Casamino acids, 1 g Bacto Yeast Extract (Difco), 150 g NaCl, 15 g agar, and 1 L artificial seawater adjusted to pH 7.5.
Composition
flowchart LR
CultureMech_008522["halomarina_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_008522 --> ing0_Casamino_acids
ing1_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008522 --> ing1_Bacto_Yeast_Extract__Difco_
ing2_NaCl["NaCl"]
CultureMech_008522 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar["Agar"]
CultureMech_008522 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
ing4_Artificial_seawater["Artificial seawater"]
CultureMech_008522 --> ing4_Artificial_seawater
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 1.0 G_PER_L |
FOODON:03315719
mammalian milk protein (hydrolyzed)
|
NBRC Medium 1214 lists 1.0 g/L Casamino acids. |
| 2 | Bacto Yeast Extract (Difco) | 1.0 G_PER_L |
FOODON:03315426
yeast extract
|
NBRC Medium 1214 lists 1.0 g/L Bacto Yeast Extract (Difco). |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
150.0 G_PER_L |
CHEBI:26710
sodium chloride
|
NBRC Medium 1214 lists 150.0 g/L NaCl. |
| 4 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
NBRC Medium 1214 lists 15.0 g/L Agar. |
| 5 | Artificial seawater | 1.0 L | NBRC Medium 1214 lists artificial seawater as the solvating medium for the Halomarina medium recipe. |
Preparation
- Prepare the medium from 1 g Casamino acids, 1 g Bacto Yeast Extract, 150 g NaCl, 15 g agar, and 1 L artificial seawater.
- Adjust pH to 7.5.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.711757Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1943 |
| 2026-01-28T19:48:25.102721Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:57.723295Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008522 |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.346068+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Halomarina medium" to "halomarina_medium" for consistent matching |
| 2026-06-05T07:41:07.842078+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.719857+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-09-10T00:00:00-07:00 | repair_halomarina_jcm703_score15.py | RESOLVED_HALOMARINA_JCM703_SCORE15 | NBRC Medium 1214 records Halomarina medium as 1 g Casamino acids, 1 g Bacto Yeast Extract (Difco), 150 g NaCl, 15 g agar, and 1 L artificial seawater adjusted to pH 7.5. |