Overview
- External ontology
- TOGO:M1948 — BG11
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1948 Original source: NBRC - NBRC_M1221 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1221
Composition
flowchart LR
CultureMech_008527["bg11"]
ing0_1M_HEPES_KOH["1M HEPES-KOH"]
CultureMech_008527 --> ing0_1M_HEPES_KOH
ing1_Distilled_water["Distilled water"]
CultureMech_008527 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008527 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008527 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_K2HPO4["K2HPO4"]
CultureMech_008527 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Na2CO3["Na2CO3"]
CultureMech_008527 --> ing5_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing5_Na2CO3 -.-> CHEBI_29377
ing6_NaNO3["NaNO3"]
CultureMech_008527 --> ing6_NaNO3
CHEBI_63005["CHEBI:63005"]
ing6_NaNO3 -.-> CHEBI_63005
ing7_Citric_acid_H2O["Citric acid·H2O"]
CultureMech_008527 --> ing7_Citric_acid_H2O
CHEBI_31404["CHEBI:31404"]
ing7_Citric_acid_H2O -.-> CHEBI_31404
ing8_Ferric_ammonium_citrate["Ferric ammonium citrate"]
CultureMech_008527 --> ing8_Ferric_ammonium_citrate
CHEBI_31604["CHEBI:31604"]
ing8_Ferric_ammonium_citrate -.-> CHEBI_31604
ing9_Na2EDTA["Na2EDTA"]
CultureMech_008527 --> ing9_Na2EDTA
CHEBI_64734["CHEBI:64734"]
ing9_Na2EDTA -.-> CHEBI_64734
ing10_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008527 --> ing10_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing10_Na2MoO4_2H2O -.-> CHEBI_75213
ing11_H3BO3["H3BO3"]
CultureMech_008527 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008527 --> ing12_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_4H2O -.-> CHEBI_86368
ing13_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008527 --> ing13_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing13_ZnSO4_7H2O -.-> CHEBI_32312
ing14_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008527 --> ing14_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing14_CuSO4_5H2O -.-> CHEBI_31440
ing15_Co_NO3_2_6H2O["Co(NO3)2·6H2O"]
CultureMech_008527 --> ing15_Co_NO3_2_6H2O
CHEBI_86214["CHEBI:86214"]
ing15_Co_NO3_2_6H2O -.-> CHEBI_86214
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | 1M HEPES-KOH | 20 G_PER_L | Role: Buffer; Properties: Defined component, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
201.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 100.0, 100.0] |
| 3 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
38 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
39 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Na2CO3
CO3.2Na 105.988 g/mol
|
20 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NaNO3
NO3.Na 84.994 g/mol
|
1.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Citric acid·H2O
C6H8O7.H2O 210.138 g/mol
|
6.3 G_PER_L |
CHEBI:31404
Citric acid monohydrate
|
Properties: Defined component, Organic compound, Simple component [Merged 2 duplicates: 6.0, 0.3] |
| 9 | Ferric ammonium citrate | 0.3 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component |
| 10 |
Na2EDTA
C10H14N2O8.2Na 336.208 g/mol
|
0.5 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Simple component, Organic compound, Defined component |
| 11 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
39 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
286 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
181 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
22.2 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
7.9 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
Co(NO3)2·6H2O
Co.6H2O.2NO3 291.031 g/mol
|
4.9 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
Stock solution1*
Dose: 2 G_PER_L
Trace A5+Co**
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.722827Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1948 |
| 2026-01-28T19:48:07.092756Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:11.439595+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:38.163861Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:57.775637Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008527 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.399123+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BG11" to "bg11" for consistent matching |
| 2026-05-16T09:03:11.898704+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.038231+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:25.093871+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.452766+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |