Overview
- External ontology
- TOGO:M1954 — PDA +Hygromycin medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1954 Original source: NBRC - NBRC_M1232 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1232
Composition
flowchart LR
CultureMech_008534["pda_hygromycin_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008534 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glucose["Glucose"]
CultureMech_008534 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_Agar["Agar"]
CultureMech_008534 --> ing2_Agar
CHEBI_2509["CHEBI:2509"]
ing2_Agar -.-> CHEBI_2509
ing3_Potato["Potato"]
CultureMech_008534 --> ing3_Potato
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 3 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 4 | Potato | 200 G_PER_L | Properties: Undefined component |
Solutions
Sodium hygromycin solution (50 mg/ml)*
Dose: 0.8 G_PER_L
Role: Antimicrobial agent; Properties: Organic compound, Solution, Defined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.737405Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1954 |
| 2026-01-28T19:48:07.026273Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:11.332127+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:57.842726Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008534 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:39.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.468112+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PDA +Hygromycin medium" to "pda_hygromycin_medium" for consistent matching |
| 2026-05-16T09:04:28.473566+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.999336+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |