benzoate_medium

CultureMech:008537 bacterial COMPLEX SOLID_AGAR
Original name: Benzoate medium

Overview

External ontology
TOGO:M1957 — Benzoate medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1957 Original source: NBRC - NBRC_M1234 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1234

Composition

flowchart LR
    CultureMech_008537["benzoate_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008537 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008537 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008537 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008537 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KH2PO4["KH2PO4"]
    CultureMech_008537 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5__NH4_2_HPO4["(NH4)2·HPO4"]
    CultureMech_008537 --> ing5__NH4_2_HPO4
    CHEBI_63051["CHEBI:63051"]
    ing5__NH4_2_HPO4 -.-> CHEBI_63051
    ing6_Agar_Noble__Difco_["Agar Noble (Difco)"]
    CultureMech_008537 --> ing6_Agar_Noble__Difco_

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
950 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.2 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 0.5 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
5 KH2PO4
H2O4P.K 136.084 g/mol
1.2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
6 (NH4)2·HPO4
2H4N.HO4P 132.056 g/mol
3 G_PER_L CHEBI:63051
diammonium hydrogen phosphate
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 Agar Noble (Difco) 20 G_PER_L Role: Solidifying component; Properties: Defined component

Solutions

Sodium benzoate solution (3 g/50 ml)*

Dose: 50 G_PER_L

Properties: Defined component, Organic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.741086Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1957
2026-01-28T19:48:28.218892Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:46.720206+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:57.859855Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008537
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.486561+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Benzoate medium" to "benzoate_medium" for consistent matching
2026-05-16T09:03:11.922885+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:35.066241+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:25.121023+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:48.460835+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.