bn_agar

CultureMech:008539 bacterial COMPLEX SOLID_AGAR
Original name: BN agar

Overview

External ontology
TOGO:M1959 — BN agar
Applications
  • Microbial cultivation
Notes
TOGO M1959 / NBRC Medium 1236 lists 13 g Nutrient Broth (OXOID), 1.44 g sodium benzoate, 250 mg kanamycin, 1 L distilled water, and 15 g agar if needed per liter; pH is unadjusted. The NBRC footnote marks the starred sodium benzoate and kanamycin rows for separate filter sterilization before addition to the autoclaved base.

Composition

flowchart LR
    CultureMech_008539["bn_agar"]
    ing0_Nutrient_Broth__OXOID_["Nutrient Broth (OXOID)"]
    CultureMech_008539 --> ing0_Nutrient_Broth__OXOID_
    ing1_Sodium_benzoate["Sodium benzoate"]
    CultureMech_008539 --> ing1_Sodium_benzoate
    CHEBI_113455["CHEBI:113455"]
    ing1_Sodium_benzoate -.-> CHEBI_113455
    ing2_Kanamycin["Kanamycin"]
    CultureMech_008539 --> ing2_Kanamycin
    CHEBI_6104["CHEBI:6104"]
    ing2_Kanamycin -.-> CHEBI_6104
    ing3_Distilled_water["Distilled water"]
    CultureMech_008539 --> ing3_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing3_Distilled_water -.-> CHEBI_15377
    ing4_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008539 --> ing4_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing4_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (5)

# Ingredient Concentration Ontology Notes
1 Nutrient Broth (OXOID) 13.0 G_PER_L TOGO M1959 / NBRC Medium 1236 lists 13.0 g/L Nutrient Broth (OXOID); retained as an intentionally opaque commercial product.
2 Sodium benzoate
C7H5O2.Na 144.105 g/mol
1.44 G_PER_L CHEBI:113455
sodium benzoate
 TOGO M1959 / NBRC Medium 1236 lists 1.44 g/L Sodium benzoate; the NBRC footnote marks it for separate filter sterilization.
3 Kanamycin 250.0 MG_PER_L CHEBI:6104
kanamycin
 TOGO M1959 / NBRC Medium 1236 lists 250.0 mg/L Kanamycin; the NBRC footnote marks it for separate filter sterilization.
4 Distilled water
H2O 18.015 g/mol
1000.0 ML_PER_L CHEBI:15377
water
 TOGO M1959 / NBRC Medium 1236 lists 1000.0 ml/L Distilled water.
5 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 TOGO M1959 / NBRC Medium 1236 lists 15.0 g/L agar if needed for solid medium.

Preparation

  1. Suspend Nutrient Broth (OXOID) and optional agar in distilled water; the source records pH as unadjusted.
  2. Sterilize the OXOID Nutrient Agar/Broth base by autoclaving.
  3. Sterilize sodium benzoate and kanamycin separately by filtration.
  4. Aseptically add filter-sterilized sodium benzoate and kanamycin to the autoclaved base.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.743896Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1959
2026-01-28T19:48:10.798050Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:17.667360+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:57.871128Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008539
2026-03-14T21:06:52.652769 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.498047+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BN agar" to "bn_agar" for consistent matching
2026-06-05T07:40:51.284281+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-11T00:00:00-07:00 repair_togo_m1959_bn_agar_score15.py RESOLVED_TOGO_M1959_BN_AGAR_SCORE15 Verified TOGO M1959 against NBRC Medium 1236, converted the imported sodium benzoate and kanamycin solution wrappers into direct filter-sterilized ingredients, grounded those selective agents, recorded the pH as unadjusted, and repaired the Nutrient Agar/Broth supplemented-variant relationship.