Overview
- External ontology
- TOGO:M1973 — Medium for Strain KKC1
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1973 Original source: NBRC - NBRC_M1251 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1251
Composition
flowchart LR
CultureMech_008555["medium_for_strain_kkc1"]
ing0_Distilled_water["Distilled water"]
CultureMech_008555 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008555 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008555 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008555 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008555 --> ing4_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_2H2O -.-> CHEBI_86158
ing5_KH2PO4["KH2PO4"]
CultureMech_008555 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_NH4Cl["NH4Cl"]
CultureMech_008555 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008555 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_H3BO3["H3BO3"]
CultureMech_008555 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_KCl["KCl"]
CultureMech_008555 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_NaHCO3["NaHCO3"]
CultureMech_008555 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_008555 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_NaNO3["NaNO3"]
CultureMech_008555 --> ing12_NaNO3
CHEBI_63005["CHEBI:63005"]
ing12_NaNO3 -.-> CHEBI_63005
ing13_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008555 --> ing13_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing13_Sodium_pyruvate -.-> CHEBI_50144
ing14_NaBr["NaBr"]
CultureMech_008555 --> ing14_NaBr
CHEBI_63004["CHEBI:63004"]
ing14_NaBr -.-> CHEBI_63004
ing15_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_008555 --> ing15_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing15_SrCl2_6H2O -.-> CHEBI_36385
ing16_KI["KI"]
CultureMech_008555 --> ing16_KI
CHEBI_8346["CHEBI:8346"]
ing16_KI -.-> CHEBI_8346
ing17_MES["MES"]
CultureMech_008555 --> ing17_MES
CHEBI_39010["CHEBI:39010"]
ing17_MES -.-> CHEBI_39010
ing18_Na2SiO3["Na2SiO3"]
CultureMech_008555 --> ing18_Na2SiO3
ing19_N2["N2"]
CultureMech_008555 --> ing19_N2
CHEBI_17997["CHEBI:17997"]
ing19_N2 -.-> CHEBI_17997
ing20_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008555 --> ing20_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_2H2O -.-> CHEBI_75213
ing21_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008555 --> ing21_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing21_FeSO4_7H2O -.-> CHEBI_75836
ing22_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008555 --> ing22_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing22_MnCl2_4H2O -.-> CHEBI_86368
ing23_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008555 --> ing23_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing23_CoCl2_6H2O -.-> CHEBI_35696
ing24_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008555 --> ing24_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_6H2O -.-> CHEBI_34887
ing25_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008555 --> ing25_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing25_ZnSO4_7H2O -.-> CHEBI_32312
ing26_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008555 --> ing26_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing26_CuCl2_2H2O -.-> CHEBI_86318
ing27_EDTA["EDTA"]
CultureMech_008555 --> ing27_EDTA
CHEBI_4735["CHEBI:4735"]
ing27_EDTA -.-> CHEBI_4735
ing28_Biotin["Biotin"]
CultureMech_008555 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008555 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_008555 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1002.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1000.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.9 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
16 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.14 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.1 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3.9 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
30.0 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Collapsed 2 identical duplicates: 30.0, 30.0] |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.65 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NaNO3
NO3.Na 84.994 g/mol
|
0.05 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 15 |
NaBr
Br.Na 102.894 g/mol
|
0.1 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
15 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
KI
I.K 166.002 g/mol
|
0.05 G_PER_L |
CHEBI:8346
potassium iodide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | MES | 1 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Organic compound, Defined component, Simple component |
| 19 | Na2SiO3 | 10 G_PER_L | Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component | |
| 20 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 21 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
3 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
20 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
2 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
10 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
1 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
Properties: Defined component, Organic compound, Simple component |
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace mineral solution*
Dose: 10 G_PER_L
See mediadive_5445_Trace_mineral_solution.yaml for composition
Vitamin solution**
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.763442Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1973 |
| 2026-01-28T19:48:12.154508Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-02-06T05:10:18.504746Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-09T08:04:20.055465+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:48.008257Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:57.983150Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008555 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:16.606789+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Strain KKC1" to "medium_for_strain_kkc1" for consistent matching |
| 2026-05-16T09:04:20.509205+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.636268+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.183064+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:58:21.448177+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:06.257650+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-02T07:38:48.233071+00:00 | repair_merged_duplicates.py | REPAIRED_SUMMED_DUPLICATE_MERGE | Collapsed 1 ingredient row(s) whose concentration was the SUM of identical duplicate values written by cleanup_media_quality.py. Every merged part was the same value, so the pre-merge value is unambiguous and no source lookup was needed. Rows whose merged parts differ were left untouched. |