lb_desferrioxamine_b

CultureMech:008564 bacterial COMPLEX SOLID_AGAR
Original name: LB + Desferrioxamine B

Overview

External ontology
TOGO:M1981 — LB + Desferrioxamine B
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M1981 imports NBRC Medium 1265 LB + Desferrioxamine B. NBRC 1265 lists the LB base with 65 mg/L Deferoxamine mesylate salt, 15 g/L agar if needed, and pH 7.0.

Composition

flowchart LR
    CultureMech_008564["lb_desferrioxamine_b"]
    ing0_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
    CultureMech_008564 --> ing0_Bacto_Tryptone__Difco_
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008564 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008564 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Deferoxamine_mesylate_salt["Deferoxamine mesylate salt"]
    CultureMech_008564 --> ing3_Deferoxamine_mesylate_salt
    CHEBI_4356["CHEBI:4356"]
    ing3_Deferoxamine_mesylate_salt -.-> CHEBI_4356
    ing4_Distilled_water["Distilled water"]
    CultureMech_008564 --> ing4_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing4_Distilled_water -.-> CHEBI_15377
    ing5_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008564 --> ing5_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Bacto Tryptone (Difco) 10 G_PER_L NBRC Medium 1265 lists Bacto Tryptone (Difco) as a source-disclosed digest product not reducible to one ChEBI molecule.
2 Yeast extract 5 G_PER_L FOODON:03315426
yeast extract
 TOGO M1981 / NBRC Medium 1265 lists 5 g/L Yeast extract.
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 TOGO M1981 / NBRC Medium 1265 lists 5 g/L NaCl.
4 Deferoxamine mesylate salt
C25H48N6O8 560.693 g/mol
65 MG_PER_L CHEBI:4356
desferrioxamine B
 NBRC Medium 1265 specifies 65 mg/L Deferoxamine mesylate salt. ChEBI has a direct descriptor for the active desferrioxamine B compound.
5 Distilled water
H2O 18.015 g/mol
1000 ML_PER_L CHEBI:15377
water
 TOGO M1981 / NBRC Medium 1265 lists 1000 ml/L Distilled water.
6 Agar (if needed) 15 G_PER_L CHEBI:2509
agar
 TOGO M1981 / NBRC Medium 1265 lists 15 g/L agar if needed.

Preparation

  1. Prepare the LB base from 10 g/L Bacto Tryptone (Difco), 5 g/L yeast extract, 5 g/L NaCl, 65 mg/L Deferoxamine mesylate salt, 1000 ml/L distilled water, and 15 g/L agar if needed.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.790588Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1981
2026-01-28T19:48:29.054271Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:58.108741Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008564
2026-03-17T06:30:27.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:16.734763+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB + Desferrioxamine B" to "lb_desferrioxamine_b" for consistent matching
2026-06-05T07:41:10.857128+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 2 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-09-12T00:00:00-07:00 repair_togo_m1981_lb_desferrioxamine_score15.py RESOLVED_TOGO_M1981_LB_DESFERRIOXAMINE_SCORE15 TOGO M1981 imports NBRC Medium 1265 LB + Desferrioxamine B. NBRC 1265 lists the LB base with 65 mg/L Deferoxamine mesylate salt, 15 g/L agar if needed, and pH 7.0. Corrected the imported distilled-water row from a g/L artifact to an ml/L volume and converted the Deferoxamine mesylate salt dose from g/L to mg/L.