Overview
- External ontology
- TOGO:M19 — R Agar
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: TOGO Medium M19 | Link: https://togomedium.org/medium/M19 TOGO Medium M19 mirrors JCM Medium 26 R Agar: peptone and extract nutrients, glycerol, 50 mg/L Tween 80, magnesium sulfate heptahydrate, agar, and distilled water, with a final pH of 7.2.
Composition
flowchart LR
CultureMech_008585["r_agar"]
ing0_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_008585 --> ing0_Bacto_peptone__BD_Difco_
ing1_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008585 --> ing1_Yeast_extract__BD_Difco_
ing2_Malt_extract__BD_Difco_["Malt extract (BD-Difco)"]
CultureMech_008585 --> ing2_Malt_extract__BD_Difco_
ing3_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_008585 --> ing3_Casamino_acids__BD_Difco_
ing4_Beef_extract__BD_Difco_["Beef extract (BD-Difco)"]
CultureMech_008585 --> ing4_Beef_extract__BD_Difco_
ing5_Glycerol["Glycerol"]
CultureMech_008585 --> ing5_Glycerol
CHEBI_17754["CHEBI:17754"]
ing5_Glycerol -.-> CHEBI_17754
ing6_Tween_80["Tween 80"]
CultureMech_008585 --> ing6_Tween_80
CHEBI_53426["CHEBI:53426"]
ing6_Tween_80 -.-> CHEBI_53426
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_008585 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_Agar["Agar"]
CultureMech_008585 --> ing8_Agar
CHEBI_2509["CHEBI:2509"]
ing8_Agar -.-> CHEBI_2509
ing9_Distilled_water["Distilled water"]
CultureMech_008585 --> ing9_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing9_Distilled_water -.-> CHEBI_15377
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Bacto peptone (BD-Difco) | 10 G_PER_L |
MICRO:0000178 (source link unavailable)
Bacto peptone
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 2 | Yeast extract (BD-Difco) | 5 G_PER_L |
FOODON:03315426
yeast extract
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 3 | Malt extract (BD-Difco) | 5 G_PER_L |
FOODON:03301056
malt extract
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 4 | Casamino acids (BD-Difco) | 5 G_PER_L | JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. | |
| 5 | Beef extract (BD-Difco) | 2 G_PER_L |
FOODON:03302088
beef extract
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 6 |
Glycerol
C3H8O3 92.094 g/mol
|
2 G_PER_L |
CHEBI:17754
glycerol
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 7 |
Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
|
0.05 G_PER_L |
CHEBI:53426
polysorbate 80
|
JCM Medium 26 lists 50 mg/L Tween 80. |
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 9 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
JCM Medium 26 lists this component for R Agar; the salinity variants reuse the same agar base. |
| 10 |
Distilled water
H2O 18.015 g/mol
|
1000 ML_PER_L |
CHEBI:15377
water
|
JCM Medium 26 brings the R Agar recipe to 1 L with distilled water. |
Preparation
- Adjust pH to 7.2.
- Autoclave at 121 C for 15 min.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.580403Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M19 |
| 2026-01-28T19:48:28.209122Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.393745Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008585 |
| 2026-03-17T06:30:25.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.012959+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R Agar" to "r_agar" for consistent matching |
| 2026-06-05T07:40:35.212436+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.580881+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T06:57:25.255522+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-09-06T00:00:00-07:00 | repair_bacterial_togo_score40.py | LINKED_BACTERIAL_TOGO_SCORE40_CHILDREN | Added or refreshed reciprocal parent-child link for bacterial/TOGO_M109_R_Agar_With_Catalase.yaml. |
| 2026-09-06T00:00:00-07:00 | repair_bacterial_togo_score40.py | LINKED_BACTERIAL_TOGO_SCORE40_CHILDREN | Added or refreshed reciprocal parent-child link for bacterial/TOGO_M1159_R_Agar_pH_9.0.yaml. |
| 2026-09-08T00:00:00-07:00 | repair_r_agar_salinity_score20.py | RESOLVED_R_AGAR_SALINITY_SCORE20 | Rebuilt the R Agar base from the JCM Medium 26 table. |
| 2026-09-08T00:00:00-07:00 | repair_r_agar_salinity_score20.py | LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN | Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M105_R_Agar_With_3_NaCl.yaml. |
| 2026-09-08T00:00:00-07:00 | repair_r_agar_salinity_score20.py | LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN | Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M67_R_Agar_With_5_NaCl.yaml. |
| 2026-09-08T00:00:00-07:00 | repair_r_agar_salinity_score20.py | LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN | Added or refreshed reciprocal supplemented-variant link for bacterial/TOGO_M297_R_Agar_With_0.5_Tween_80.yaml. |
| 2026-09-08T00:00:00-07:00 | repair_r_agar_salinity_score20.py | LINKED_R_AGAR_SALINITY_SCORE20_CHILDREN | Added or refreshed reciprocal salinity-variant link for bacterial/TOGO_M916_R_Agar_With_10_NaCl.yaml. |