Overview
- External ontology
- TOGO:M2002 — SL medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2002 Original source: NBRC - NBRC_M1289 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1289
Composition
flowchart LR
CultureMech_008589["sl_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008589 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008589 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008589 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008589 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008589 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_008589 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_Resazurin["Resazurin"]
CultureMech_008589 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008589 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008589 --> ing8_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_9H2O -.-> CHEBI_76209
ing9_KCl["KCl"]
CultureMech_008589 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Sodium_acetate["Sodium acetate"]
CultureMech_008589 --> ing10_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing10_Sodium_acetate -.-> CHEBI_32954
ing11_Maltose["Maltose"]
CultureMech_008589 --> ing11_Maltose
CHEBI_18167["CHEBI:18167"]
ing11_Maltose -.-> CHEBI_18167
ing12_PIPES["PIPES"]
CultureMech_008589 --> ing12_PIPES
CHEBI_39033["CHEBI:39033"]
ing12_PIPES -.-> CHEBI_39033
ing13_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008589 --> ing13_Bacto_Yeast_Extract__Difco_
ing14_Bacto_Peptone__Difco_["Bacto Peptone (Difco)"]
CultureMech_008589 --> ing14_Bacto_Peptone__Difco_
ing15_NaOH["NaOH"]
CultureMech_008589 --> ing15_NaOH
CHEBI_32145["CHEBI:32145"]
ing15_NaOH -.-> CHEBI_32145
ing16_N2["N2"]
CultureMech_008589 --> ing16_N2
CHEBI_17997["CHEBI:17997"]
ing16_N2 -.-> CHEBI_17997
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
26 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.35 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.35 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Defined component, Inorganic compound, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1.6 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 12 |
Maltose
C12H22O11 342.297 g/mol
|
3.5 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 13 | PIPES | 3.4 G_PER_L |
CHEBI:39033
PIPES
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 14 | Bacto Yeast Extract (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 15 | Bacto Peptone (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 16 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 17 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.853529Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2002 |
| 2026-01-28T19:48:37.143996Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-13T06:19:58.443814Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008589 |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.061366+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SL medium" to "sl_medium" for consistent matching |
| 2026-05-16T09:03:12.108028+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:35.254140+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.522980+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |