Overview
- External ontology
- TOGO:M2015 — Difco Nutrient Agar/Broth
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- TOGO M2015 imports NBRC Medium 1304, which defines Difco Nutrient Agar/Broth per liter as 8.0 g Nutrient Broth (Difco), 1.0 L Distilled water, 15.0 g Agar if needed, and pH 7.0. BD catalog 234000 identifies Nutrient Broth as a BD Difco dehydrated culture medium.
Composition
flowchart LR
CultureMech_008602["difco_nutrient_agar_broth"]
ing0_Nutrient_Broth__Difco_["Nutrient Broth (Difco)"]
CultureMech_008602 --> ing0_Nutrient_Broth__Difco_
ing1_Distilled_water["Distilled water"]
CultureMech_008602 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Agar__if_needed_["Agar (if needed)"]
CultureMech_008602 --> ing2_Agar__if_needed_
CHEBI_2509["CHEBI:2509"]
ing2_Agar__if_needed_ -.-> CHEBI_2509
Ingredients (3)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Nutrient Broth (Difco) | 8.0 G_PER_L | NBRC Medium 1304 lists 8.0 g/L Nutrient Broth (Difco). This commercial BD-Difco base is retained as a source-disclosed opaque component. | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1.0 L |
CHEBI:15377
water
|
NBRC Medium 1304 lists 1.0 L distilled water. |
| 3 | Agar (if needed) | 15.0 G_PER_L |
CHEBI:2509
agar
|
NBRC Medium 1304 lists 15.0 g/L agar for solid medium when needed. |
Preparation
- Mix 8.0 g/L Nutrient Broth (Difco) with 1.0 L distilled water; add 15.0 g/L agar when preparing solid medium.
- Adjust pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.879318Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2015 |
| 2026-01-28T19:48:26.989803Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.563506Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008602 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.181016+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Difco Nutrient Agar/Broth" to "difco_nutrient_agar_broth" for consistent matching |
| 2026-06-05T07:40:58.707067+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-09-12T00:00:00-07:00 | repair_togo_m2015_difco_nutrient_score15.py | RESOLVED_TOGO_M2015_DIFCO_NUTRIENT_SCORE15 | TOGO M2015 imports NBRC Medium 1304, which defines Difco Nutrient Agar/Broth per liter as 8.0 g Nutrient Broth (Difco), 1.0 L Distilled water, 15.0 g Agar if needed, and pH 7.0. BD catalog 234000 identifies Nutrient Broth as a BD Difco dehydrated culture medium. Corrected the imported water unit artifact, added the NBRC pH, grounded water and optional agar, and kept Nutrient Broth (Difco) intentionally unmapped as a source-disclosed commercial base. |