Overview
- External ontology
- TOGO:M2020 — Medium for Strain KC3
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2020 Original source: NBRC - NBRC_M1311 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1311
Composition
flowchart LR
CultureMech_008608["medium_for_strain_kc3"]
ing0_Distilled_water["Distilled water"]
CultureMech_008608 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4__7H2O["MgSO4 ·7H2O"]
CultureMech_008608 --> ing1_MgSO4__7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4__7H2O -.-> CHEBI_31795
ing2_Yeast_extract["Yeast extract"]
CultureMech_008608 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_008608 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_K2HPO["K2HPO"]
CultureMech_008608 --> ing4_K2HPO
ing5_Resazurin["Resazurin"]
CultureMech_008608 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_CaCO3["CaCO3"]
CultureMech_008608 --> ing6_CaCO3
CHEBI_3311["CHEBI:3311"]
ing6_CaCO3 -.-> CHEBI_3311
ing7_Cellobiose["Cellobiose"]
CultureMech_008608 --> ing7_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing7_Cellobiose -.-> CHEBI_17057
ing8_Cysteine_HCl["Cysteine HCl"]
CultureMech_008608 --> ing8_Cysteine_HCl
ing9_nitrogen_gas["nitrogen gas"]
CultureMech_008608 --> ing9_nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_nitrogen_gas -.-> CHEBI_17997
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
MgSO4 ·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 | Yeast extract | 0.1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 5 | K2HPO | 1 G_PER_L | Role: Buffer; Properties: Simple component, Inorganic compound, Defined component | |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 7 |
CaCO3
CO3.Ca 100.086 g/mol
|
0.2 G_PER_L |
CHEBI:3311
calcium carbonate
|
Role: Protective agent, Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 8 |
Cellobiose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:17057
cellobiose
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 9 | Cysteine HCl | 0.5 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 10 |
nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Hipolypepton*
Dose: 0.5 G_PER_L
Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.891708Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2020 |
| 2026-01-28T19:48:17.596215Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: nitrogen gas |
| 2026-03-09T08:04:29.123017+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:58.624281Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008608 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.246284+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Strain KC3" to "medium_for_strain_kc3" for consistent matching |
| 2026-05-16T09:04:20.491871+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.618417+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:21.430158+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |