Overview
- External ontology
- TOGO:M2021 — AM3 medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M2021 Original source: NBRC - NBRC_M1312 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1312
Composition
flowchart LR
CultureMech_008609["am3_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008609 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008609 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008609 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KH2PO4["KH2PO4"]
CultureMech_008609 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_K2HPO4["K2HPO4"]
CultureMech_008609 --> ing4_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing4_K2HPO4 -.-> CHEBI_131527
ing5_Glucose["Glucose"]
CultureMech_008609 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_008609 --> ing6_Agar__if_needed_
ing7_Beef_extract["Beef extract"]
CultureMech_008609 --> ing7_Beef_extract
ing8_Peptone["Peptone"]
CultureMech_008609 --> ing8_Peptone
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 1.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.32 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.68 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
1 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 8 | Beef extract | 1.5 G_PER_L | Properties: Undefined component | |
| 9 | Peptone | 5 G_PER_L |
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.893353Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2021 |
| 2026-01-28T19:48:29.051014Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:58.630349Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008609 |
| 2026-03-17T06:30:27.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.252597+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AM3 medium" to "am3_medium" for consistent matching |
| 2026-06-05T07:40:48.337095+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.968424+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-09-11T00:00:00-07:00 | repair_togo_m2023_am3_antibiotics_score15.py | LINKED_TOGO_M2023_AM3_ANTIBIOTICS_VARIANT | Linked TOGO M2023 / NBRC Medium 1314 as a supplemented variant of AM3 medium. |