am3_nalidixic_acid_kanamycin_medium (CultureMech:008611)

Overview

External ontology

TOGO:M2023 — AM3 + Nalidixic acid, Kanamycin medium

Applications

Microbial cultivation

Notes

NBRC Medium 1314 defines AM3 + Nalidixic acid, Kanamycin medium with 1.5 g beef extract, 1.5 g yeast extract, 5 g peptone, 1 g glucose, 3.5 g NaCl, 3.68 g K2HPO4, 1.32 g KH2PO4, 15 g agar if needed, 1 L distilled water, 1 ml Nalidixic acid solution (100 mg/ml), and 1 ml Kanamycin solution (25 mg/ml) per liter. The pH is unadjusted; the antibiotic solutions are sterilized separately by filtration.

Composition

flowchart LR
    CultureMech_008611["am3_nalidixic_acid_kanamycin_medium"]
    ing0_Beef_extract["Beef extract"]
    CultureMech_008611 --> ing0_Beef_extract
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008611 --> ing1_Yeast_extract
    ing2_Peptone["Peptone"]
    CultureMech_008611 --> ing2_Peptone
    ing3_Glucose["Glucose"]
    CultureMech_008611 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_NaCl["NaCl"]
    CultureMech_008611 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_K2HPO4["K2HPO4"]
    CultureMech_008611 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_KH2PO4["KH2PO4"]
    CultureMech_008611 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008611 --> ing7_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar__if_needed_ -.-> CHEBI_2509
    ing8_Distilled_water["Distilled water"]
    CultureMech_008611 --> ing8_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing8_Distilled_water -.-> CHEBI_15377

Ingredients (9)

#	Ingredient	Concentration	Ontology	Notes
1	Beef extract	1.5 G_PER_L	FOODON:03302088 Beef extract	TOGO M2023 / NBRC Medium 1314 lists 1.5 g/L Beef extract.
2	Yeast extract	1.5 G_PER_L	FOODON:03315426 yeast extract	TOGO M2023 / NBRC Medium 1314 lists 1.5 g/L Yeast extract.
3	Peptone	5.0 G_PER_L	MICRO:0000178 (source link unavailable) Peptone	TOGO M2023 / NBRC Medium 1314 lists 5 g/L Peptone.
4	Glucose C6H12O6 180.156 g/mol	1.0 G_PER_L	CHEBI:17234 glucose	TOGO M2023 / NBRC Medium 1314 lists 1 g/L Glucose.
5	NaCl Cl.Na 58.443 g/mol	3.5 G_PER_L	CHEBI:26710 sodium chloride	TOGO M2023 / NBRC Medium 1314 lists 3.5 g/L NaCl.
6	K2HPO4 HO4P.2K 174.174 g/mol	3.68 G_PER_L	CHEBI:131527 dipotassium hydrogen phosphate	TOGO M2023 / NBRC Medium 1314 lists 3.68 g/L K2HPO4.
7	KH2PO4 H2O4P.K 136.084 g/mol	1.32 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	TOGO M2023 / NBRC Medium 1314 lists 1.32 g/L KH2PO4.
8	Agar (if needed)	15.0 G_PER_L	CHEBI:2509 agar	TOGO M2023 / NBRC Medium 1314 lists 15 g/L Agar if needed; retained for the solid agar preparation.
9	Distilled water H2O 18.015 g/mol	1000.0 ML_PER_L	CHEBI:15377 water	TOGO M2023 / NBRC Medium 1314 lists 1 L Distilled water per liter.

Preparation

Mix beef extract, yeast extract, peptone, glucose, NaCl, K2HPO4, KH2PO4, and agar in distilled water.
Autoclave the basal AM3 medium.
Filter-sterilize the Nalidixic acid solution (100 mg/ml) and Kanamycin solution (25 mg/ml) separately.
Aseptically add 1.0 ml/L Nalidixic acid solution and 1.0 ml/L Kanamycin solution to the autoclaved basal medium.

Solutions

Nalidixic acid solution (100 mg/ml)

Dose: 1.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Nalidixic acid	100.0 MG_PER_ML	NBRC Medium 1314 specifies this stock as 100.0 mg/ml Nalidixic acid.

TOGO M2023 / NBRC Medium 1314 lists 1 ml/L Nalidixic acid solution (100 mg/ml).

Kanamycin solution (25 mg/ml)

Dose: 1.0 ML_PER_L

Stock composition
Ingredient	Concentration	Notes
Kanamycin	25.0 MG_PER_ML	NBRC Medium 1314 specifies this stock as 25.0 mg/ml kanamycin.

TOGO M2023 / NBRC Medium 1314 lists 1 ml/L Kanamycin solution (25 mg/ml).

Target organisms

No target organisms are recorded.

Provenance

Timestamp	Curator	Action	Notes
2026-01-27T06:35:58.896129Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M2023
2026-01-28T19:48:12.621574Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:20.842819+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 2 solution(s) from ingredients
2026-03-13T06:19:58.643263Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008611
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name
2026-03-17T06:29:52.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.265424+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "AM3 + Nalidixic acid, Kanamycin medium" to "am3_nalidixic_acid_kanamycin_medium" for consistent matching
2026-05-16T09:03:23.952835+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=1
2026-06-05T07:40:48.344874+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.971713+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-11T00:00:00-07:00	repair_togo_m2023_am3_antibiotics_score15.py	RESOLVED_TOGO_M2023_AM3_ANTIBIOTICS	Verified TOGO M2023 against NBRC Medium 1314, corrected distilled water to 1000 ml/L, converted the nalidixic acid and kanamycin imports to separately filter-sterilized 1 ml/L stock solutions, grounded the AM3 base and antibiotic rows, recorded the pH as unadjusted, and changed the AM3 relationship from source duplicate to supplemented variant.