basal_medium_formaldehyde

CultureMech:008615 bacterial COMPLEX SOLID_AGAR
Original name: Basal medium + Formaldehyde

Overview

External ontology
TOGO:M2027 — Basal medium + Formaldehyde
Applications
  • Microbial cultivation
pH
7.0
Notes
TOGO M2027 imports NBRC Medium 1318 as Basal medium + Formaldehyde. NBRC 1318 lists 1 g K2HPO4, 10 g Hipolypepton from Wako Pure Chemical Industries, 5 g yeast extract, 5 g NaCl, 0.5 ml Formaldehyde, 1 L distilled water, optional 15 g agar, and pH 7.0, and states that Formaldehyde is sterilized separately by filtration.

Composition

flowchart LR
    CultureMech_008615["basal_medium_formaldehyde"]
    ing0_K2HPO4["K2HPO4"]
    CultureMech_008615 --> ing0_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing0_K2HPO4 -.-> CHEBI_131527
    ing1_Hipolypepton_["Hipolypepton*"]
    CultureMech_008615 --> ing1_Hipolypepton_
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008615 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008615 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Formaldehyde__["Formaldehyde**"]
    CultureMech_008615 --> ing4_Formaldehyde__
    CHEBI_16842["CHEBI:16842"]
    ing4_Formaldehyde__ -.-> CHEBI_16842
    ing5_Distilled_water["Distilled water"]
    CultureMech_008615 --> ing5_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing5_Distilled_water -.-> CHEBI_15377
    ing6_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008615 --> ing6_Agar__if_needed_
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar__if_needed_ -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 K2HPO4
HO4P.2K 174.174 g/mol
1.0 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 NBRC Medium 1318 lists 1.0 g/L K2HPO4.
2 Hipolypepton* 10.0 G_PER_L NBRC Medium 1318 lists 10 g/L Hipolypepton and notes that the asterisk marks Wako Pure Chemical Industries; this peptone product is retained as an opaque complex component.
3 Yeast extract 5.0 G_PER_L FOODON:03315426
yeast extract
 NBRC Medium 1318 lists 5.0 g/L Yeast extract.
4 NaCl
Cl.Na 58.443 g/mol
5.0 G_PER_L CHEBI:26710
sodium chloride
 NBRC Medium 1318 lists 5.0 g/L NaCl.
5 Formaldehyde**
CH2O 30.026 g/mol
0.5 ML_PER_L CHEBI:16842
formaldehyde
 NBRC Medium 1318 lists 0.5 ml/L Formaldehyde and notes that the double asterisk marks separate filter sterilization.
6 Distilled water
H2O 18.015 g/mol
1.0 L CHEBI:15377
water
 NBRC Medium 1318 lists 1 L distilled water.
7 Agar (if needed) 15.0 G_PER_L CHEBI:2509
agar
 NBRC Medium 1318 lists 15.0 g/L Agar (if needed).

Preparation

  1. Sterilize Formaldehyde separately by filtration.

Target organisms

No target organisms are recorded.

Provenance

TimestampCuratorActionNotes
2026-01-27T06:35:58.901802Z togo-import Imported from TOGO Medium Source: NBRC, ID: M2027
2026-01-28T19:48:08.474841Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:13.713934+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:58.668882Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008615
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:42.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:17.291294+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Basal medium + Formaldehyde" to "basal_medium_formaldehyde" for consistent matching
2026-05-16T09:03:25.458051+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:50.533763+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:53.805106+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-09-12T00:00:00-07:00 repair_togo_m2025_m2027_aldehyde_basal_score15.py RESOLVED_TOGO_M2025_M2027_SCORE15 TOGO M2027 imports NBRC Medium 1318 as Basal medium + Formaldehyde. NBRC 1318 lists 1 g K2HPO4, 10 g Hipolypepton from Wako Pure Chemical Industries, 5 g yeast extract, 5 g NaCl, 0.5 ml Formaldehyde, 1 L distilled water, optional 15 g agar, and pH 7.0, and states that Formaldehyde is sterilized separately by filtration. Moved Formaldehyde and Hipolypepton out of empty solution wrappers, corrected liquid quantities, and marked the NBRC formula as curated.