Overview
- External ontology
- TOGO:M2028 — Medium for Strain HC45 (Anaerobic cultivation)
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M2028 Original source: NBRC - NBRC_M1319 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1319
Composition
flowchart LR
CultureMech_008616["medium_for_strain_hc45_anaerobic_cultivation"]
ing0_Distilled_water["Distilled water"]
CultureMech_008616 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008616 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008616 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008616 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008616 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_008616 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_008616 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008616 --> ing7_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing7_MgCl2_6H2O -.-> CHEBI_86345
ing8_KCl["KCl"]
CultureMech_008616 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_Na2SO4["Na2SO4"]
CultureMech_008616 --> ing9_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing9_Na2SO4 -.-> CHEBI_32149
ing10_Glucose["Glucose"]
CultureMech_008616 --> ing10_Glucose
CHEBI_17234["CHEBI:17234"]
ing10_Glucose -.-> CHEBI_17234
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008616 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_008616 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008616 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_008616 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008616 --> ing15_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_7H2O -.-> CHEBI_75836
ing16_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008616 --> ing16_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_4H2O -.-> CHEBI_86368
ing17_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008616 --> ing17_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing17_CoCl2_6H2O -.-> CHEBI_35696
ing18_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008616 --> ing18_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing18_NiCl2_6H2O -.-> CHEBI_34887
ing19_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008616 --> ing19_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing19_ZnSO4_7H2O -.-> CHEBI_32312
ing20_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008616 --> ing20_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_2H2O -.-> CHEBI_86318
ing21_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
CultureMech_008616 --> ing21_HCl__25___7_7_M_
ing22_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_008616 --> ing22_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_5H2O -.-> CHEBI_131361
ing23_Na2WO4_2H2O["Na2WO4·2H2O"]
CultureMech_008616 --> ing23_Na2WO4_2H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_2H2O -.-> CHEBI_63939
ing24_NaOH["NaOH"]
CultureMech_008616 --> ing24_NaOH
CHEBI_32145["CHEBI:32145"]
ing24_NaOH -.-> CHEBI_32145
ing25_NaHCO3["NaHCO3"]
CultureMech_008616 --> ing25_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing25_NaHCO3 -.-> CHEBI_32139
ing26_CO2_gas["CO2 gas"]
CultureMech_008616 --> ing26_CO2_gas
ing27_Sodium_phosphate_buffer__10_mM__pH7_1_["Sodium phosphate buffer (10 mM; pH7.1)"]
CultureMech_008616 --> ing27_Sodium_phosphate_buffer__10_mM__pH7_1_
ing28_Biotin["Biotin"]
CultureMech_008616 --> ing28_Biotin
CHEBI_15956["CHEBI:15956"]
ing28_Biotin -.-> CHEBI_15956
ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008616 --> ing29_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
CultureMech_008616 --> more["...8 more"]
Ingredients (38)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2251.5 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 6 duplicates: 963.5, 987.0, 1.0, 100.0, 100.0, 100.0] |
| 2 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 8 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
Na2SO4
2Na.O4S 142.043 g/mol
|
4 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
Glucose
C6H12O6 180.156 g/mol
|
1.8 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
30 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
2100 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 | HCl (25%; 7.7 M) | 12.5 G_PER_L | Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component | |
| 23 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
6 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2WO4·2H2O
2H2O.2Na.O4W 329.846 g/mol
|
8 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Simple component |
| 26 |
NaHCO3
CHO3.Na 84.006 g/mol
|
8.4 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 27 | CO2 gas | variable VARIABLE | Properties: ガス | |
| 28 | Sodium phosphate buffer (10 mM; pH7.1) | 100 G_PER_L | Role: Buffer; Properties: Inorganic compound, Solution, Defined component | |
| 29 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 30 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
4 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 31 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
15 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
10 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 | Sodium phosphate buffer (25 mM; pH 3.4) | 100 G_PER_L | Role: Buffer; Properties: Defined component, Inorganic compound, Solution | |
| 35 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
10 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Cyanocobalamin
C63H88CoN14O14P 1355.388 g/mol
|
5 G_PER_L |
CHEBI:17439
cyanocob(III)alamin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
24 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 38 | N2 gas | variable VARIABLE | Properties: ガス |
Solutions
Trace element mixture*
Dose: 1 G_PER_L
Selenite-tungstate solution**
Dose: 1 G_PER_L
See mediadive_5543_Selenite-tungstate_solution.yaml for composition
Bicarbonate solution (1.0 M)***
Dose: 30 G_PER_L
Vitamin solution****
Dose: 1 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Thiamine solution*****
Dose: 1 G_PER_L
See mediadive_4133_Thiamine_solution.yaml for composition
Vitamin B12 solution******
Dose: 1 G_PER_L
See mediadive_6105_Vitamin_B12_solution.yaml for composition
Sulfide solution (1.0 M)*******
Dose: 1.5 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.902984Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M2028 |
| 2026-01-28T19:48:16.897072Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: CO2 gas; Added default concentration for ingredient: N2 gas |
| 2026-03-09T08:04:27.926718+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T03:08:56.677688Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 5 duplicate ingredient(s) |
| 2026-03-13T06:19:58.686076Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008616 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:17.308161+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Strain HC45 (Anaerobic cultivation)" to "medium_for_strain_hc45_anaerobic_cultivation" for consistent matching |
| 2026-05-16T09:04:20.354597+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:29.481074+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:47.153187+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T07:47:06.197139+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |